杂拟谷盗体内Wolbachia感染密度的时间和空间分布状况

The temporal and spatial distribution status of Wolbachia infection density in the confused flour beetle Tribolium confusum

为搞清杂拟谷盗体内沃尔巴克氏体(Wolbachia)感染密度的时间和空间分布状况,本研究采用实时荧光定量PCR方法测定了杂拟谷盗不同发育阶段、不同日龄、不同性别和不同身体部位的Wolbachia感染密度。结果表明,杂拟谷盗在卵和成虫期Wolbachia感染密度高于其幼虫和蛹期,成虫腹部的Wolbachia感染密度高于其头部和胸部,而成虫不同日龄和雌雄之间的Wolbachia感染密度均没有显著性差异。本研究明确了Wolbachia在杂拟谷盗体内的时空分布和动态变化规律,这对于揭示寄主与共生菌之间的互作关系有着重要意义。

To clarify the spatiotemporal distribution status of Wolbachia infection density in the confused flour beetle Tribolium confusum（ Jacquelin du Val）,we determined Wolbachia infection density in host different developmental stages,ages,sexes and body parts by the real-time fluorogenic quantitative PCR.The results showed that Wolbachia infection density was higher in eggs and adults than in larvae and pupae;Wolbachia infection density in adult abdomen was higher than in both head and thorax. However,Wolbachia infection density in adults did not differ between males and females and among different ages. All in all,observations on Wolbachia spatiotemporal infection density dynamics in T. confusum help to improve the understanding ofinteraction relationships between Wolbachia and host.