BOLD-MRI评价肾透明细胞癌HIF-α表达的可行性研究

Study on the Evaluation of the Correlation between the Expression Level of HIF in Clear Cell Renal Cell Carcinoma and R2＊ Values in BOLD-MRI

目的评价透明细胞肾癌（CRCC）R2＊值变化与肿瘤组织内缺氧诱导因子（HIF-1α、HIF-2α）表达的相关性。方法回顾性分析26例经手术病理证实的CRCC患者血氧水平依赖MRI与HIF-1α、HIF-2α的结果。使用Furhman法划分肿瘤的病理级别,并将所有患者分成低级别和高级别两个亚组。测量所有患者肿瘤实质的R2＊值。比较高低级别组CRCC之间HIF-1α、HIF-2α表达情况的差异。使用Pearson相关分析法评价R2＊值与HIF-α阳性表达率之间的关系。结果低级别组（Ⅰ＋Ⅱ）17例,高级别组（Ⅲ＋Ⅳ）9例。高级别组R2＊值明显高于低级别组（P〈0.005）。高级别组HIF-α阳性表达率值明显高于低级别组（P〈0.05）。CRCC的R2＊值与HIF-2α表达与组织的R2＊值呈正相关（P〈0.05）。结论 R2＊值作为一种无创的评价指标有助于CRCC的病理核分级,且R2＊值与HIF-2α表达水平存在相关性。

Objective To evaluate the correlation between R2＊values of various pathological grades of clear cell renal cell carcinoma（ CRCC） and the expression levels of intratumoral hypoxia inducible factors（ HIF-1α、HIF-2α）. Methods Blood-oxygen-level dependent MRI images and the expression levels of HIF-1αand HIF-2α of 26 patients with CRCC,who all underwent surgical operations and were confirmed by pathological examinations were collated. All tumors were classified according to the Furhman criterion and then they were categorised into low-grade and high-grade group. R2＊values of the tumor parenchyma were measured. The differences of the expression levels of HIF-1αand HIF-2αbetween low-grade and high-grade CRCCs were compared. Pearson correlation coefficient was used to evaluate the correlations between R2＊values of CRCCs and the positive expression level of HIF-α. Results Low-grade group（ Grade Ⅰ ＋ Ⅱ） constituted 17 cases and high-grade group（ Grade Ⅲ ＋ Ⅳ） constituted 9 cases. R2＊values of high-grade tumors were significantly higher than those of low-grade tumors. The positive expression levels of high-grade tumors were higher than those of low-grade tumors（ P 〈 0. 05）. R2＊values of CRCCs positively correlated with the expression levels of HIF-2α. Conclusion R2＊values can be used as a non-invasive index to evaluate CRCC grades and it correlates with the expression level of HIF-2α.