不同乳酸菌亚油酸异构酶酶学性质的比较

Characterization of linoleic acid isomerase from different Lactobacillus

对不同乳酸菌菌种中提取出的亚油酸异构酶进行了酶学性质的比较。结果表明,嗜酸乳杆菌CGMCC1.1854亚油酸异构酶的最适p H为6.5,最适温度为30℃,以亚油酸为底物时酶的米氏常数Km为18.99mmo L·L-1,Vmax为1.58μg·m L-1·min-1;植物乳杆菌CGMCC 1.557亚油酸异构酶的最适p H为6.5,最适温度为45℃,以亚油酸为底物时酶的米氏常数Km为14.83 mmo L·L-1,Vmax为1.75μg·m L-1·min-1;植物乳杆菌3-9亚油酸异构酶的最适p H为6.5,最适温度为45℃,以亚油酸为底物时酶的米氏常数Km为14.26 mmo L·L-1,Vmax为1.94μg·m L-1·min-1。

In this paper, the attributes of linoleic acid isomerase from different lactobacillus were compared. The results showed that the optimum p H for Lactobacillus acidophilus CGMCC1.1854 linoleic acid isomerase was 6.5 and the optimum temperature was about 30℃, The apparent Km and Vmax of the enzyme was 18.99 mmo L·L-1and 1.58 μg·m L-1·min-1 when the substrate was linoleic acid..The optimum p H for Lactobacillus plantarum CGMCC1.557 linoleic acid isomerase was 6.5 and the optimum temperature was about 45℃, the apparent Kmand Vmax of the enzyme was 14.83 mmo L·L-1and 1.75 μg·m L-1·min-1 when the substrate was linoleic acid. The optimum p H for Lactobacillus plantarum 3-9 linoleic acid isomerase was 6.5 and the optimum temperature was about 45℃, the apparent Km and Vmax of the enzyme was 14.26 mmo L·L-1and 1.94 μg·m L-1·min-1 when the substrate was linoleic acid.