籽瓜色素的稳定性及其清除自由基能力的研究

Study on the Stability and Free Radical Scavenging Ability of Seed-used Watermelon Pigment

目的:研究籽瓜瓤中色素的稳定性及其清除自由基能力。方法:将籽瓜色素添加在氧化性、还原性、食品添加剂、金属离子中,对其稳定性及清除羟自由基、抗超氧阴离子自由基及总抗氧化能力(T-AOC)进行试验。结果:籽瓜色素在一定范围内,耐氧化性较好,在还原剂及葡萄糖中稳定性好,在柠檬酸中稳定性较差,Fe^(3+),Fe^(2+)对该色素均有增色作用,Fe^(3+)的影响最大,Zn^(2+),Cu^(2+),Al ^(3+),Na^+均有降色作用,Zn^(2+)降色影响较大,Cu^(2+),Al ^(3+),Na^+影响较小。抑制羟自由基1.268U/mg prot,抗超氧自由基能力18U/g prot,每分钟每毫克色素粗提物的总抗氧化活性0.238单位。结论:籽瓜色素具有良好的清除自由基活性,稳定性较好,可作为一种天然抗氧化活性色素来源。

Objective：Study the stability and free radicals scavenging ability of seed-used watermelon pigment.Method：The seed-used watermelon pigment is added into the oxidation,reduction,food additives,metal ions for testing its stability and abilities of free radicals scavenging,superoxide anion free radicals and T-AOC.Result：The antioxidative activity of seed-used watermelon is better within a certain range;it possesses good stability in reductant and glucose except citric acid.Fe^3＋and Fe^2＋have a considerable effect on color increasing of the pigment except Zn^2＋,Cu^2＋,Al^3＋,Na^＋,among which,Zn^2＋has a great influence on color decreasing,Cu^2＋,Al^3＋,Na^＋have a little influence.Inhibit hydroxyl free radicals of 1.268U/mg prot,inhibit super oxide free radical of 18U/g prot,the total antioxidant activity per minute per mg pigment crude extract is 0.238 units.Conclusion：The seed-used watermelon pigment has good activity of scavenging free radicals,better stability,can be used as a pigment source of natural antioxidant activity.