冬性白菜型油菜的遗传多样性分析

Analysis of the Genetic Diversity of Brassica rapa L.

利用15对SSR引物，对51份冬性白菜型油菜种质资源进行了遗传多样性分析。共检测到96条多态性条带，多态性比率为100％；平均每对引物检测到6．4个等位变异，PIC变幅为0．5834．0．8740，平均为0．7794。51份种质资源间遗传相似系数0．500-0．969。聚类分析表明，在相似系数0．63处，51份种质资源被分为2个类群，第一类包含33个品种（系），第二类包含18个品种（系）。每个类群又可以分为多个不同的亚簇。来源于甘肃省内的材料间表现出较大的遗传差异，说明甘肃省冬性白菜型油菜具有非常丰富的遗传多样性，这种来源于特殊地域的种质能够为白菜型油菜的育种提供优良基因资源。

This study has analysised the genetic diversity of 51 winter rapeseed(Brassica rapa L.) varietie. A total of 96 polymorphic bands are detected by 15 pairs of SSR markers. The polymorphic fragment percentage is 100%. Average number of polymorphic fragments per pair of primer were 6.4,genetic diversity index ranged 0.583 4~0.874 0 with an average of 0.779 4. The similarity coefficient is rage from 0.500 to 0.969 between the two randomly accessions. Clustering result of SSR showed that 51 accessions are divided into 2 groups,with the 1th group contain 33 accessions and the 2nd group contain 18 accessions. Both of the2 groups are divided into different subgroups. The conclusion is that a high level of genetic diversity is identified among all the 51 accessions,especially in the accessions come from Tianshui(Gansu province). And these germplasm maybe provide excellent gene resources for the rapeseed(Brassica rapa L.)breeding.