金边碧玉组培快繁技术研究

Study on Tissue Culture and Rapid Propagation of Peperomia tetraphylla

以金边碧玉的叶片为外植体,对其组织培养与快繁技术进行了初步研究。试验结果表明,适宜叶片直接诱导丛生芽的培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,诱导出的丛生芽粗壮嫩绿,继代培养基为MS+6-BA1.0 mg/L+NAA 0.1 mg/L和MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,叶片愈伤组织诱导培养基为MS+6-BA3.0mg/L+NAA 0.5mg/L,最佳生根培养基为1/2MS+IBA 0.8 mg/L和1/2MS+IBA 1.0 mg/L。

Using leaves of Peperomia tetraphylla as explants,culture and rapid propagation are studied on the microstructure. The experiment indicated that the suitable leaf direct induction of multiple shoots culture medium is MS +6-BA1.0 mg/L +NAA 0.1 mg/L and MS +6-BA 1.0 mg/L +NAA 0.2 mg/L,with induced shoots stout green. and leaf callus induction medium is MS+6BA 3.0 mg/L+NAA 0.5 mg/L,The best rooting medium is 1/2 MS+IBA 0.8 mg/L and 1/2 MS+IBA 1.0 mg/L.