非诺贝特对卵形鲳鲹PPARα及CPTI基因表达的影响

Effects of Fenofibrate on Relatively Expression of Gene PPAR α and CPTI

对卵形鲳鲹(Trachinotus ovatus)腹腔注射20、50、100 mg/kg的激动剂非诺贝特,用RT-PCR检测肝脏、肌肉中PPARα和CPTI基因表达的变化趋势,研究激动剂对卵形鲳鲹PPARα和CPTI基因表达的影响。结果表明,PPARα基因表达量50 mg/kg非诺贝特组高于其他组,差异有统计学意义(P<0.05),而CPTI表达量在20 mg/kg组有较高的表达水平(P<0.05);50 mg/kg非诺贝特组中PPARα基因表达量随着时间的变化呈现先降低后恢复至初始水平的趋势,CPTI表达量随时间变化先升高后降低,随后又升高的变化趋势,肝脏组织中,CPTI表达量在注射后30 h达到最高(P<0.05),而在肌肉组织中18 h时达到最高值(P<0.05);非诺贝特组PPARα和CPTI基因的表达量均高于PBS对照组(P<0.05)。非诺贝特可诱导PPARα基因的表达,但CPTI与PPARα基因表达无较明显的相关性。

Thefenofibrate solution with concentration of 20, 50,100 mg/kg were injected into the abdomenofTrachinotus ovatus respectively, and the expression of genePPAR alpha andCPTIin livers and muscles were analyzed by RT-PCR, and the effects oftheagonistson relativelyexpression ofPPARαandCPTI gene was studied.Theresult showed that the expression ofPPAR α in group 50 mg/kg is significant higher than that in other groups(P<0.05), andCPTIhad higher expression in group 20mg/kg (P<0.05).The expression ofPPARαandCPTI gene had no significant difference in PBS group(P>0.05), but the expression ofPPAR α in group 50 mg/kgfirstly increased and decreased and thenreturned to the initial level trend finally as time changed.Meantime,the&nbsp;expression ofCPTIgenefell after rose and then rose at lastas the time gone by.The highest expression appeared at 30h afterinjection in livers and the highest expression appeared at 18h in muscles(P<0.05).Theexpression of genePPAR alphain livers and muscles withfenofibrate solution is higher than thatwith PBS.In conclusion,the fenofibrate can inducethe expression of PPAR α, but thecorrelation of the expression betweenPPAR α andCPTIisnot obviously.