丹参毛状根和丹参药材HPLC指纹图谱的比较研究

Comparative Study on HPLC Fingerprints of Salvia Miltiorrhiza Hairy Roots and Salvia Miltiorrhiza

为了分析丹参毛状根和丹参原药材之间有效成分的差异，探讨丹参毛状根作为丹参药材使用的可行性，利用不同诱导处理后的丹参毛状根的HPLC指纹图谱进行研究。结果表明：ABA处理后的丹参毛状根有效成分提高了1．3倍，但与药材指纹图谱相似性较差，相似性系数仅为0．30，仅可以作为单一有效成分的提取原料；与原药材相比，MJ处理的丹参毛状根有效成分将近提高了15倍，指纹图谱相似性也较高，相似性系数达到0．96。因此，MJ处理的毛状根具有作为丹参药材使用的可行性。

To analyze effective component differences of S.miltiorrhizahairy roots and S.miltiorrhiza and explore the feasibility of S.miltiorrhiza hairy roots as S.miltiorrhiza,HPLC Fingerprints of S.miltiorrhiza hairy roots with different induction treatments were investigated.The results indicated that the effective components of S.miltiorrhiza hairy roots with ABA treatment were increased by 1.3folds.However,the fingerprinting similarity between hairy foots and S.miltiorrhiza raw material was poor and the similarity coefficient was only 0.30,so it could only be used as an extractive raw material of a single effective component.Under MJ treatment,the effective component of hairy roots was almost increased by 15 folds when compared with the raw material.The fingerprinting similarity was high and similarity coefficient reached to 0.96.Therefore,hairy roots treated by MJ has the feasibility to be used as S.miltiorrhiza material.