黄芩苷对肝脏缺血再灌注损伤大鼠诱生型一氧化氮合酶、核转录因子和Caspase-3表达的影响

Effects of Baicalin on i NOS, NF-κB and Caspase-3 in Liver Ischemia Reperfusion Injury Rats

目的观察黄芩苷对肝脏缺血再灌注损伤大鼠肝组织形态、肝细胞凋亡、诱生型一氧化氮合酶(i NOS)和核因子(NF)-κB m RNA表达及Caspase-3蛋白表达的影响,探讨其作用机制。方法采用Pringle's法建立大鼠肝脏缺血再灌注模型。实验大鼠随机分为假手术组、模型组、黄芩苷组,假手术组、模型组给予生理盐水灌胃,黄芩苷组给予黄芩苷灌胃。观察大鼠肝脏组织病理形态,TUNEL法检测肝细胞凋亡指数,RT-PCR检测肝组织i NOS、NF-κB m RNA表达,Western blot检测Caspase-3蛋白表达。结果与假手术组比较,模型组i NOS和NF-κB m RNA表达、Caspase-3蛋白表达水平及肝细胞凋亡指数升高(P<0.05,P<0.01);与模型组比较,黄芩苷组i NOS和NF-κB m RNA表达、Caspase-3蛋白表达水平及肝细胞凋亡指数下降(P<0.05),肝损害明显改善。结论黄芩苷通过下调肝组织i NOS和NF-κB的表达、抑制Caspase-3介导的细胞凋亡,对缺血再灌注损伤大鼠肝脏发挥保护作用。

Objective To observe the effects of baicalin on forms of hepatic tissue, liver apoptosis, mRNAexpressions of iNOS, NF-κB and protein expression of Caspase-3 in rats with ischemia reperfusion injury; To discussits mechanism of action. Methods The rat models of liver ischemia reperfusion were performed according to thePringle＇s method. Rats were randomly divided into sham-operation group, model group and baicalin group.Sham-operation group and model group were given normal saline for gavage, while baicalin group was given baicalinfor gavage. Morphological characteristic was observed by HE staining. Hepatocyte apoptosis was determined byTUNEL. The mRNA expressions of iNOS and NF-κB were determined by RT-PCR. The protein expression ofCaspase-3 was determined by Western blot. Results Compared with the sham-operation group, mRNA expressions ofiNOS and NF-κB and the protein expression of Caspase-3 in the model group increased, as well as liver apoptosis rate（P〈0.05, P〈0.01）; compared with the model group, mRNA expressions of iNOS and NF-κB and the proteinexpression of Caspase-3 in the baicalin group decreased, as well as liver apoptosis rate （P〈0.05）, and the hepaticlesion significantly improved in the baicalin group. Conclusion Baicalin can restrain Caspase-3 induced apoptosis byreducing the expressions of iNOS and NF-κB, with a purpose to realize the hepatoprotective effect for liver of ratswith ischemia reperfusion injury.