大黄素对人肝癌HepG2细胞迁移侵袭能力及E-钙黏蛋白、Slug蛋白表达的影响

Effects of Emodin on Migration and Invasion Ability and Expressions of E-cadherin and Slug of Human Hepatoma Cell Lines HepG2

目的观察大黄素对人肝癌Hep G2细胞迁移侵袭能力及E-钙黏蛋白(E-cadherin)、Slug蛋白表达的影响,探讨其相关作用机制。方法体外培养Hep G2细胞,大黄素低、高剂量组分别加入10、20μmol/L大黄素,阴性对照组加入等体积RPMI-1640培养液,阳性对照组加入10μmol/L 5-氟尿嘧啶。分别采用细胞-基质黏附实验、划痕修复实验、Transwell小室体外侵袭实验观察Hep G2细胞黏附率、迁移、侵袭能力;Western blot检测E-cadherin和Slug蛋白表达。结果与阴性对照组比较,大黄素低、高剂量组显著抑制Hep G2细胞黏附率、迁移、侵袭能力,E-cadherin蛋白表达水平显著升高,Slug蛋白表达水平显著降低(P<0.05,P<0.01),并呈浓度依赖性。结论大黄素能明显抑制Hep G2细胞迁移和侵袭能力,其机制可能与增强E-cadherin及抑制Slug蛋白表达有关。

Objective To investigate the effects of emodin on the migration and invasion ability and expressions of E-cadherin and Slug of human hepatoma cell lines HepG2; To discuss the possible mechanisms of anti-hepatocellular carcinoma.Methods HepG2 cells were cultured in vitro. The experimental group was treated with emodin at concentrations of 10μmol/L and 20μmol/L as. The negative control group was treated with the same volume of RPMI-1640 medium, while the positive control group was treated with 10μmol/L floxuridine. The cell matrix adhesion assay, wound healing and transwell chamber in vitro invasion assay were used to observe the effects of emodin on HepG2 cell adhesion rate and migration and invasion ability. Western blot analysis was used to observe the changes of expressions of E-cadherin and Slug.Results Compared with the negative control group, emodin inhibited significantly HepG2 cell adhesion rate and migration and invasion ability were in a dose-dependent manner (P<0.05,P<0.01); Western blot analysis showed that the protein expression of E-cadherin increased significantly, and the level of Slug decreased significantly in a dose-dependent manner (P<0.05,P<0.01).Conclusion Emodin can significantly inhibit migration and invasion of HepG2 cells, which mechanism may up-regulate expressions of E-cadherin and down-regulate Slug.