蜘蛛香中总环烯醚萜及2个指标性成分baldrinal和11-ethoxyviburtinal含量测定

Content Determination of Total Iridoid Compounds and Baldrinal and 11-ethoxyviburtinal from Valerianae Jatamansi Rhizoma et Radix

目的 建立蜘蛛香药材中总环烯醚萜类成分及2 种环烯醚萜类成分baldrinal（缬草醛）和11-ethoxyviburtinal（环戊烷-吡喃-7-甲醛,4-乙氧基甲基）的含量测定方法,并测定3 个产地蜘蛛香药材中的成分含量.方法 采用紫外分光光度法,以11-ethoxyviburtinal 为对照品,在波长288 nm 处测定总环烯醚萜的含量;采用高效液相色谱法测定baldrinal 和11-ethoxyviburtinal 的含量,采用Phenomenex Luna C18 色谱柱,以乙腈-磷酸水为流动相,流速为0.95 mL/min,检测波长为288 nm,柱温为30 ℃.结果 蜘蛛香中总环烯醚萜类成分的浓度在2.088～14.616 μg/μL范围内线性关系良好,baldrinal、11-ethoxyviburtinal 分别在74.88～224.64 μg、41.6～249.60 μg 范围内线性关系良好,方法精密度、重复性、稳定性、加样回收率均良好.结论 本方法准确、简便、快速,可用于蜘蛛香药材的质量控制.

Objective To establish a method for content determination of total iridoid compounds and baldrinaland 11-ethoxyviburtinal from Valerianae Jatamansi Rhizoma et Radix; To determine the contents of total iridoidcompounds and baldrinal and 11-ethoxyviburtinal in Valerianae Jatamansi Rhizoma et Radix from three medicinalorigins. Methods UV spectrophotometry was applied, 11-ethoxyviburtinal （cyclopentane-pyran-7-formaldehyde,4-ethoxy methyl） was set as the reference substance, and the content of total iridoid compounds was determined at288 nm. HPLC method was used to simultaneously determine the contents of baldrinal and 11-ethoxyviburtinal. TheHPLC analysis was performed on a Phenomenex Luna C18 column （250 mm×4.6 mm, 5 μm）. The mobile phase wascomposed of acetonitrile-water in gradient elution at a flow rate of 0.95 mL/min. The detection wavelength was 288 nmand the column temperature was 30 ℃. Results The total iridoid compounds, baldrinal and 11-ethoxyviburtinal werein good linearity within the ranges of 2.088–14.616 μg/μL, 74.88–224.64 μg, and 41.6–249.6 μg, respectively. Thismethod was precise, and with good repeatability, stability and recovery rate. Conclusion The method is accurate,simple, rapid, which can be used for the quality control of Valerianae Jatamansi Rhizoma et Radix.