肺结核患者白细胞介素-24水平变化及意义

The Change and Significance of Interleukin-24 in Tuberculosis Patients

目的:探讨肺结核患者白细胞介素(IL)-24水平变化及其意义。方法:以肺结核患者117例(初治组84例,复治组33例)和健康志愿者40例为研究对象,采用酶联免疫吸附法(ELISA)和逆转录-聚合酶链反应(RT-PCR)检测受试者血浆及外周血单个核细胞(PBMC)中IL-24的水平。结果:与健康对照组比较,结核患者血浆中IL-24水平下降[(4.5.±1.26)vs(9.62±1.33)ng/ml,P<0.01];与初治组比较,复治组IL-24水平下降[(3.35±1.39)vs(4.95±0.85)ng/ml,P<0.01]。PBMC中IL-24mRNA水平显示与上述相同的变化趋势,差异有统计学意义(P<0.01)。结论:IL-24水平与肺结核的发生、发展密切相关,低IL-24水平可能是肺结核初治患者失败或治愈后复发的重要原因。

Objective:To explore the change and significance of interleukin-24(IL-24)in tuberculosis patients.Methods:Whole vein blood was obtained from the tuberculosis patients with initial treatment(n=84),re-treatment(n=33)and normal control(n=40).The level of serum IL-24 and the expression of mRNA in peripheral blood mononuclear cell(PBMC)were analysed by ELISA and RT-PCR respectively.Results:compared with healthy controls,the level of serum IL-24 and the expression of mRNA in PBMC in tuberculosis patients were lower[(4.5.±1.26)vs(9.62±1.33)ng/ml,P<0.01];compared with initial treatment group,the level of serum IL-24 in re-treatment group were lower[(3.35±1.39)vs(4.95±0.85)ng/ml,P<0.01].The expression of IL-24 mRNA in PBMC shows the same changes as above(P<0.01).Conclusion:The level of IL-24 may be closely related to the development of pulmonary tuberculosis.The low level of IL-24 may be one of influencing factors for ineffective treatment in the initial treatment and relapse patients.