接种尖镰孢菌对枸杞苯丙烷代谢关键酶及产物的影响

Effects of key enzymes and products in phenylpropanoid pathway of Lyciuminfected by Fusarium oxysporum

为了阐明抗感枸杞与根腐病病原菌互作过程中苯丙烷代谢途径中关键酶和代谢产物的变化差异与抗病性的关系,以枸杞栽培品种宁杞一号（NingqiⅠ）、宁杞二号（NingqiⅡ）,国内野生种中国枸杞、宁夏枸杞,美洲引进野生种L.brevipes、L.exsertum等6种材料为参试材料,采用切根法接种强致病菌尖镰孢菌后统计死亡率,以此筛选出抗病和感病材料,并测定二者0~20d内苯丙氨酸解氨酶（PAL）、肉桂酸羟化酶（C4H）、4-香豆酰-辅酶A连接酶（4CL）等苯丙烷代谢途径关键酶以及总酚和类黄酮等代谢产物的动态变化。结果表明,6种参试材料接种枸杞根腐病强致病菌尖镰孢菌后,野生材料较栽培材料抗病性强,宁杞一号为感病材料,L.exsertum抗根腐病能力最强;抗病材料L.exsertum苯丙烷代谢关键酶苯丙氨酸解氨酶、肉桂酸羟化酶、4-香豆酰-辅酶A连接酶活性显著高于宁杞一号,初步确定苯丙烷代谢中的苯丙氨酸解氨酶、肉桂酸羟化酶、4-香豆酰-辅酶A连接酶的活性和类黄酮的含量与枸杞材料抗根腐病呈正相关。因此,苯丙氨酸解氨酶、肉桂酸羟化酶、4-香豆酰-辅酶A连接酶活性和类黄酮含量,初步可以作为筛选抗枸杞尖镰孢菌根腐病的生化指标。

In this study, the phenylpropanoid pathway of the interaction of wolfberry and root rot pathogens will be discussed. The relationship between wolfberry phenylpropanoid pathway and disease resistance will be clarified. In this study, the experimental materials are cultivated varieties Ningqi I , Ningqi 1I ; domestic wild species Lycium chinense, L. barbarum; wild species of America L. brevipes, L. eccsertum. The strong patho genic Fusarium oxysporum isolated from the onset of wolfberry will be inoculation by root cutting method. And the anti-sense wolfbeey will be choose. This 2 kinds of wolfberry as materials to study the change of phen ylalanine ammonia lyase, cinnamic acid hydroxylase, 4-hydroxylase p-coumaric acyl-coenzyme A ligase, phe nolics and flavonoids in 20 days. The results show that the disease resistance of wild materials are strong than the cultivated material in 6 kinds of test materials which were inoculated with wolfberry root rot strong patho gen F. oxysporum. Ningqi I is susceptible materials. The resistance to root rot disease of L. eccsertum are strongest. The key enzymes of phenylpropanoid pathway like phenylalnine ammonialyase, trans-cinnamic acid 4-hydroxylase, 4-coumarate： coenzyme A ligase activity are significantly higher than Ningqi I . Initially identi fied the phenylalnine ammonialyase, trans-cinnamic acid-4-hydroxylase, 4-coumarate： coenzyme A ligase activ ity and flavonoid content of phenylpropanoid pathway of test material resistance to root rot disease was posi tively correlated. Therefore, the phenylalnine ammonialyase, trans-cinnamic acid-4-hydroxylase, 4-coumarate： coenzyme A ligase enzyme activity and flavonoid content can be a screening of anti-Chinese wolfberry Fusarium root rot of biochemical indexes preliminary.