组蛋白去乙酰化酶2在慢性阻塞性肺疾病急性加重期诊疗中的意义

The implication of HDAC2 in diagnosis and treatment patients with acute exacerbation of chronic obstructive pulmonary disease

目的探讨慢性阻塞性肺疾病急性加重期(AECOPD)患者外周血组蛋白去乙酰化酶2(HDAC2)浓度与C反应蛋白(CRP)、降钙素原(PCT)及肺功能的关系。方法选择60例AECOPD患者为研究对象,选择年龄、性别相近的40名健康体检者为对照组。测定患者治疗后0、3、7和10 d的HDAC2、CRP、PCT浓度并进行肺功能、血气分析。结果 AECOPD患者在治疗后0、3、7和10 d,外周血CRP、PCT水平均逐渐下降,而HDAC2浓度逐渐升高(P<0.05)。以上4个时间点检测的HDAC2值,与对应时间点检测的CRP、PCT浓度均呈负相关(r_(CRP)=-0.316、-0.435、-0.498和-0.547,r_(PCT)=-0.332、-0.414、-0.481和-0.523,P<0.01)。AECOPD患者HDAC2浓度与同期肺功能呈正相关(r_(FEV1)=0.594,r_(FEV1/预计值%)=0.561,P<0.01)。结论HDAC2可能是AECOPD患者炎症反应的负调控因子,且与患者气流阻塞程度相关。

Objective To explore the con＇elation among the concentration of peripheral blood histone deacetylase 2 （HDAC2）, C -reactive protein （CRP）, proealeitonin （PCT）and lung function among patients with acute exacerbation period of chronic obstructive pulmonary disease （AECOPD）. Methods A total of 60 patients with AECOPD were selected as the test group, and 40 healthy subjects in the same medical examination center were selected as the control group. The HDAC2, CRP,PCT,lung function testing and blood gas analysis were measured on Day 0,3,7,10. Results Along with the process of AECOPD patients,in the treatment of 0 d, 3 d, 7 d, 10 d, the average level of CRP and PCT in peripheral blood of patients was decreased gradually, while the average level of HDAC2 was gradually increased （ P 〈 0.05 ）. In patients treated with 0 d, 3 d, 7 d, 10 d, HDAC2 levels were negatively con＇elated with CRP and PCT at the same time （rcRe was - 0. 316, - 0. 435, - 0. 495, - 0. 547,respectively, and recT was - 0. 414, - 0. 332, - 0. 481, - 0. 523,respectively, P 〈0.01 ）. The level of HDAC2 was positively correlated with lung function in the same period （rFEV1was 0. 594, and r~,EVl/Expeoted valueWaS 0. 561, P 〈 0.01 ）. Conclusion HDAC2 may be a negative regulator of the inflammatory response among the patients with AECOPD, and is associated with the degree of airflow obstruction of the patients.