罗布麻叶总黄酮提取物对抑郁大鼠的作用和对S100A10表达的影响

Antidepressant effect of flavonoids extracted from Apocynum venetum leaves and expression of S100A10 in rat model of depression

目的研究罗布麻叶总黄酮提取物对慢性应激抑郁大鼠的抗抑郁作用,并探讨其对S100A10基因表达的影响。方法将105只SD大鼠随机分为7组,每组15只,根据给药成分与剂量分为对照组、抑郁组,低、高剂量预防组(分别给予罗布麻叶总黄酮提取物40、80 mg/kg),低、高剂量治疗组(分别给予罗布麻叶总黄酮提取物40、80 mg/kg)和氟西汀组(氟西汀20 mg/kg)。采用慢性不可预知性轻度应激(CUMS)法建立大鼠抑郁模型,通过旷场实验观察各组大鼠垂直运动次数及水平运动总路程,采用实时荧光定量PCR方法检测大鼠海马区S100A10 mRNA表达。结果与对照组相比,抑郁组、罗布麻叶总黄酮提取物各干预组和氟西汀组大鼠在应激4周后旷场实验中的水平运动总路程及垂直活动次数减少(P<0.05);与抑郁组相比,罗布麻叶总黄酮提取物高预防组和高治疗组的垂直运动次数,及罗布麻叶总黄酮提取物各干预组的水平运动总路程显著增加(P<0.05);氟西汀组垂直运动次数及水平运动总路程比抑郁组增加(P<0.05)。与对照组相比,抑郁组大鼠海马组织S100A10基因表达水平升高,罗布麻叶总黄酮提取物低预防组和低治疗组S100A10基因表达水平均降低(P<0.05);与抑郁组相比,罗布麻叶总黄酮提取物各干预组和氟西汀组S100A10基因表达水平均降低(P<0.05)。结论罗布麻叶总黄酮提取物对CUMS大鼠具有抗抑郁样行为作用,调节S100A10基因表达可能是罗布麻叶总黄酮提取物抗抑郁作用机制之一。

Objective To study the antidepressant effect and underlying mechanism of flavonoids extracted from Apocynum venetum leaves （ AVL - extracts） in rat depression model. Methods The rats were randomly divided into seven parts including the control group, depression model group, low dose AVL - extracts prevention group （40 mg/kg） , high dose AVL- extracts prevention group （80 mg/kg）, low dose AVL- extracts treatment group （40 mg/kg）, high dose AVL- extracts treatment group （80 mg/kg ） and fluoxetine treatment group, respectively. The rat depression model was established by the method of chronic unpredictable mild stress （CUMS）. The open field test was used to evaluate the autonomic activities. The relative expression levels of S100A10 mRNA were detected by Real - time PCR. Results The open field activities decreased significantly in the depression model group including horizontal movements and vertical movements with comparison to the normal group （P 〈 0.05 ）. The autonomic activities increased significantly after preventive administration of high dose AVL- extracts （P 〈 0.05 ）. The antidepressant effect of AVL- extracts was similar to that of fluoxetine in increasing the autonomic activities. The S100A10 mRNA expressed in the depressive group were higher than in the control group （ P 〈 0.05 ） , and the expression levels were significantly decreased after fluoxetine and AVL- extracts administration （P 〈 0.05 ）. The high dose AVL- extracts administration group and fluoxetine group showed no significant difference in the expression levels of S100A10 mRNA when compared to the control group （P 〉 0.05 ） , whereas the levels of those in low dose AVL - extracts administration groups were significantly lower than those in the control group （ P 〈 0.05 ）. Conclusion The study suggests that AVL - extracts have antidepressant effects on rat depression model. The underling mechanism of AVL - extracts antidepressant effect may be associated with regulation of S100A10 mRNA expression.