一种新型复合碘皮肤消毒液的杀菌效果和毒性观察

Experimental observation on germicidal efficacy and toxicity of a new compound iodine disinfectant

目的观察一种新型复合碘皮肤消毒液的杀菌效果和毒性。方法采用悬液定量杀菌试验和动物试验方法,对该消毒液杀菌效果和毒性进行了实验室观察。结果该复合碘消毒液原液由有效碘含2 490mg/L、葡萄糖酸氯己定9 640mg/L与体积分数66.7%乙醇复配而成。以该复合碘消毒液原液作用0.5 min,对悬液内金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌杀灭对数值均>5.00,对悬液内白色念珠菌的杀灭对数值>4.00。小鼠急性经口毒性试验LD50值>5 000 mg/kg·bw;其原液对家兔完整皮肤刺激指数为0,对小鼠骨髓嗜多染红细胞无致微核作用。结论该复合碘消毒液能快速杀灭细菌繁殖体和真菌,属于无毒级物质,对完整皮肤无刺激,无致微核作用。

Objective To observe the germicidal efficacy and toxicity of a new compound iodine disinfectant.Methods Suspension quantitative germicidal test and animal toxicity test were used to observe the germicidal efficacy and toxicity.Results The new compound iodine disinfectant contained iodine 2 490 mg / L and chlorhexidine gluconate 9 640 mg / L and ethanol 66.7%（ V / V）.The killing logarithm values of Staphylococcus aureus,Escherichia coil and Pseudomonas aeruginosa in suspension exposed to this compound iodine disinfectant for 0.5 min were all 〉5.00,while that of Candida albicans was 〉4.00.The killing logarithm of natural bacteria on the skin wiped with this compound disinfectant for 1 min was 1.0.The LD50 of mouse acute oral toxicity was 〉5 000 mg / kg·bw and the irritation test of intact skin＇s score was 0.The mouse bone marrow micro nucleus test was negative.Conclusion The compound iodine disinfectant has good killing effect on vegetative forms of bacteria and fungi.It is practically no toxic,no irritation and does not induce micronuclens form ation.