摘要
目的检测FOXC2在结直肠癌(CRC)细胞和组织中的表达,探讨FOXC2的异常表达与结直肠癌发生、发展的关系及其临床意义。方法蛋白免疫印迹法和实时定量PCR法检测FOXC2在结直肠癌细胞、结直肠癌活检组织及相应癌旁正常组织中蛋白和mRNA表达水平的差异;采用免疫组化方法检测102例结直肠癌石蜡组织切片中FOXC2的表达,并分析其与结直肠癌的临床病理因素的关系。结果 FOXC2蛋白和mRNA在具有高转移潜能的HCT116和SW620结直肠癌细胞株中均高表达,在低转移潜能的HT29细胞株中低表达,且结直肠癌组织中FOXC2蛋白和mRNA表达水平显著高于正常结直肠黏膜组织;此外,FOXC2的表达水平与Dukes分期、T分期以及远处转移呈正相关性(P<0.05)。结论 FOXC2在结直肠癌中高表达,其表达水平与结直肠癌进展密切相关,可作为预测结直肠癌进展和转移的分子标记物之一。
Purpose To investigate the expression and clinicopathologic significance of FOXC2 in colorectal cancer.Methods FOXC2 protein and mRNA expression in colorectal cancer（ CRC） cell lines,CRC biopsies and their matched adjacent tissues were detected by real time RT-PCR and Western blot,respectively. Immunohistochemincal analysis was used to examine the expression of FOXC2 in 102 cases of paraffin-embedded colorectal cancer tissues. Results FOXC2 protein and mRNA were highly expressed in aggressive colorectal cancer cell lines HCT116 and SW620,but relatively lower expressed in less aggressive cell line HT29. In addition,both of FOXC2 mRNA and protein expression levels were significantly higher in colorectal cancer biopsies than that in their matched adjacent tissues. FOXC2 was strongly expressed in paraffin-embedded colorectal cancer tissues. Additionally,strong expression of FOXC2 was significantly associated with Dukes stage,T classification and distant metastasis（ P〈0. 05）. Conclusion FOXC2 is highly expressed in colorectal cancer. High expression of FOXC2 is closely related to progression of CRC. FOXC2 may serve as a novel molecular marker for prediction of colorectal cancer progression and metastasis.
出处
《诊断病理学杂志》
CSCD
2016年第4期272-275,共4页
Chinese Journal of Diagnostic Pathology
基金
国家自然科学基金项目(81172055)
广州市珠江科技新星专项项目(2012J2200052)
