复合酶法提取褐藻酸钠的工艺研究

Study on Extraction Process of of Sodium Alginate by Composite Enzyme Method

用复合酶法提取海带中的褐藻酸钠,并对工艺进行优化。通过对单因素实验和正交试验结果的分析,确定褐藻酸钠最佳提取工艺为:粉碎的海带经过浸泡过滤后,加入海带干重1%(m/m)的纤维素酶和1%(m/m)的果胶酶,55℃(p H值4.5)水解2h,再加入1%(m/m)的木瓜蛋白酶,85℃(p H值8.5)水解2 h。此工艺的提取率高,产品纯度高,是对海带多糖传统提纯工艺的革新。

The method of compound enzymes was used to extract sodium alginate from brown seaweeds,with its process optimized. The analysis of the results of single-factor and orthogonal tests indicated that the optimum conditions of sodium alginate extraction were obtained as follows： after soaking and filtering of the dusty brown seaweeds,1%（ m / m） cellulase and1%（ m / m） pectinase（ at 55℃ and pH 4. 5） were added to extract for 2 h,followed by 1%（ m / m） protease（ at 85℃ and pH 8. 5） for 2h. The compound enzymatic extraction has advantages of high extraction ratio and fine purity quotient,which becomes an innovation of the traditional extraction- purification processes of polysaccharide in the brown seaweeds.