姜黄素联合紫杉醇在体内外可协同降低乳腺癌MDA-MB-231细胞迁移能力并降低MMP9表达

Curcumin combined with paclitaxel can reduce breast cancer MDA-MB-231 cell migration synergistically and reduce MMP9 expression in vitro and in vivo

目的:观察姜黄素联合紫杉醇对乳腺癌MDA-MB-231细胞迁移侵袭能力的抑制作用及对MMP9体内外表达的影响。方法:采用细胞划痕实验检测姜黄素联合紫杉醇对MDA-MB-231细胞迁移的影响;采用Western blotting检测MDA-MB-231细胞和MDA-MB-231细胞裸鼠移植瘤组织中MMP9蛋白的表达;实时荧光定量PCR检测MMP9 mRNA的表达。结果:在体外细胞实验中,姜黄素10μmol/L组、紫杉醇0.001μmol/L组可分别降低MDA-MB-231细胞的迁移能力,并可降低MMP9蛋白和mRNA的表达,10μmol/L姜黄素和0.0005μmol/L紫杉醇联合药物组可协同减弱细胞的迁移能力并降低MMP9的表达。裸鼠移植瘤模型实验中,姜黄素100mg/kg组、紫杉醇10mg/kg组,可分别降低MMP9蛋白和mRNA的表达,姜黄素100 mg/kg与紫杉醇5mg/kg联合组可协同降低其表达。结论:姜黄素联合紫杉醇在体内外可协同降低乳腺癌MDA-MB-231细胞迁移侵袭能力。

Objective： To observe the inhibitory effect of curcumin combined with paclitaxel on breast cancer MDA-MB-231 cells and the effect on matrix metalloproteinase 9（ MMP9） expressions in vivo and in vitro. Methods： Using Scratch test to observe the capacity of migration in breast cancer cell,western blotting was applied to examine the protein of MMP9 in cell and xenografted tissues,Real-time quantitative PCR was used to detect MMP9 mRNA. Results： In vitro experiments,curcumin 10μmol / L group,paclitaxel 0. 001μmol / L group,reduce the migratory ability of MDA-MB-231 cells respectively,and reduce the protein and mRNA expression of MMP9,10μmol / L curcumin combined with 0. 0005μmol / L paclitaxel group synergistically decreased cell migration and reduce the expression of MMP9. Xenograft tumor model experiments,curcumin 100 mg / kg group,paclitaxel 10 mg / kg groups,reduce protein and mRNA expression of MMP9 respectively. curcumin100 mg / kg combined with paclitaxel 5mg / kg group reduce its expression synergistically. Conclusion： Curcumin combined with paclitaxel in vivo and in vitro synergistically to reduce breast cancer MDA-MB-231 cell invasive ability.