一般人群尿中8-羟基脱氧鸟苷水平及其影响因素

Urinary 8-OHd G Levels and Related Impact Factors in General Population

[目的]探讨一般人群尿8-羟基脱氧鸟苷（8-OHd G）水平,以及可能影响因素的作用。[方法]选择华东某市的城市居民共257名作为研究对象,采用超高效液相色谱-串联质谱方法测定尿中8-OHd G水平,高效液相色谱法检测尿中肌酐的浓度,校正尿中的8-OHd G的浓度。影响因素分析采用多重线性回归分析,交互作用采用多因素方差分析模型分析。[结果]该地区一般人群尿中的8-OHd G的质量浓度M（P25～P75）为1.50（0.39～2.24）μg/L,肌酐校正后M（P25～P75）为129.82（50.79～235.58）μg/mol。经吸烟、饮酒情况和体质指数（BMI）分层后,没有发现吸烟、饮酒和BMI单独对尿中8-OHd G改变的影响。多重线性回归分析结果显示,在排除其他混杂因素的影响后,饮酒对尿中8-OHd G水平有影响（P=0.040）。进一步分析发现超重人群中随年龄升高8-OHd G水平有升高趋势（Ptrend〈0.05）。[结论]年龄与BMI的交互作用是尿中8-OHd G水平的重要影响因素,尤其是高年龄的超重者更易发生DNA氧化损伤。

[Objective] To measure urinary 8-hydroxy-2＇-deoxyguanosine（8-OHd G） levels in general population and assess the roles of potential impact factors. [Methods] The urinary levels of 8-OHd G of 257 urban residents in a city of east China were measured with ultra-high performance liquid chromatography-mass spectrometer, and creatinine by high performance liquid chromatography to adjust the urinary 8-OHd G levels. The impact factors were analyzed by multivariate linear regression models, and interaction effects by multivariate analysis of variance. [Results] The median（P25～P75） level of 8-OHd G in local general population was 1.50（0.39-2.24） μg/L, and the level adjusted by creatinine in urine was 129.82（50.79-235.58） μg/mol. We did not find changes of urinary 8-OHd G by smoking, drinking, and body mass index（BMI） alone after stratification analyses. By multivariate linear regression analysis, we found that drinking had a significant effect on the urinary levels of 8-OHd G（P=0.040） after controlling other selected confounding factors. And we found an increasing trend of urinary levels of 8-OHd G with older age in the overweight group（Ptrend〈0.05）. [Conclusion] The interaction between age and BMI might be an important impact factor for urinary levels of 8-OHd G. Overweight individuals in older age group tend to be at a higher risk of DNA oxidative damage.