OXTR基因在甲状腺癌组织中的甲基化状态

OXTR Gene Methylation in Thyroid Cancer Tissues

[目的]研究甲状腺癌组织与癌旁正常组织OXTR基因甲基化状态并进行比较,初步探讨OXTR基因甲基化修饰与其表达之间的关系。[方法]利用甲基化特异性PCR(MSP)技术扩增甲状腺癌患者癌组织与癌旁组织DNA甲基化(M)和非甲基化(U)OXTR基因各17例,比较两组甲基化程度差异。[结果]MSP实验结果显示,癌组织(病例组17例)中有11例呈现OXTR基因甲基化条带扩增,其余为OXTR非甲基化条带扩增,甲基化率达到64.7%;癌旁组织(对照组17例)有4例呈现OXTR基因甲基化条带扩增,13例样品皆为OXTR非甲基化条带扩增,对照组OXTR甲基化率为23.5%;两组甲基化程度差异有统计学意义(P<0.05)。[结论]OXTR基因DNA甲基化修饰程度癌组织高于癌旁组织,结合前期血液OXTR基因甲基化修饰研究,该基因甲基化可能是早期肿瘤标志,其检测结果可作为辅助诊断甲状腺癌的有效方法。

[Objective] To study and compare the thyroid cancer tissues with normal tissues adjacent to carcinoma on OXTR gene methylation status, and assess the relationship of OXTR gene methylation modification with its expression. [Methods] Methylation-specific PCR（MSP） technology was applied to amplify methylated（M） and unmethylated（U） OXTR gene from 17 thyroid cancer tissue samples and 17 surrounding tissue samples and compare differences in methylation levels between the two groups. [Results] The MSP results showed that 11 out of the 17 cancer tissue samples presented methylated OXTR gene（methylation rate, 64.7%） and other samples presented unmethylated OXTR gene; whereas 4 and 13 out of the 17 adjacent tissue samples did so（methylation rate, 23.5%）; there had a significant difference between the two groups in the degree of methylation（P〈0.05）. [Conclusion] A higher degree of OXTR gene DNA methylation modification is identified in cancer tissues than in the tissues adjacent to carcinoma. Combined with previous studies on blood OXTR gene methylation modification, the gene methylation as an early tumor marker could be used in auxiliary diagnosis of thyroid carcinoma.