HPLC-ICP-MS法检测水中Cr(Ⅲ)和Cr(Ⅵ)

Determination of Cr(Ⅲ) and Cr(Ⅵ) in water using HPLC-ICP-MS

目的建立高效液相色谱（HPLC）和电感耦合等离子体质谱（ICP-MS）联用技术检测水中三价铬Cr（Ⅲ）和六价铬Cr（Ⅵ）的分析方法。方法样品以缓冲溶液（75 m M HNO3和0.6 m M EDTA的水溶液,氨水调p H=7）配制,75 m M HNO_3（氨水调p H=7）为流动相分离两种价态的铬。结果 Cr（Ⅲ）和Cr（Ⅵ）的方法检出限均为0.5 ng/m L,线性范围均为1.5～100 ng/m L,加标回收率和精密度均满足分析要求。结论建立的HPLC-ICP-MS检测水中Cr（Ⅲ）和Cr（Ⅵ）的方法,检出限为0.5 ng/m L,方法简单准确,适用于水中Cr（Ⅲ）和Cr（Ⅵ）的检测,并为建立生物样本如血液和尿液中Cr（Ⅲ）与Cr（Ⅵ）的检测方法建立基础。

Objective To establish a high performance liquid chromatography-inductively coupled plasma mass spectrometry method for the determination of Cr（Ⅲ） and Cr（Ⅵ） in water. Method Samples were prepared with buffer solution containing75 m M HNO_3 and 0.6 m M EDTA（p H=7）. The mobile phase was 75 m M HNO_3（p H=7）. Results The detection limits of Cr（Ⅲ） and Cr（ Ⅵ） were 0.5 ng/m L, and the linear ranges were 1.5 ～100 ng/m L. Conclusion The method was proved to be accurate and reliable for the determination of Cr（Ⅲ） and Cr（Ⅵ） in water.