NKA-9大孔树脂纯化香椿叶黄酮类物质工艺优化

Purification of Flavonoids from Toona sinensis Leaves with NKA-9 Macroporous Resin

以香椿叶提取物为原料,以吸附率和解吸率为指标,考察了9种大孔树脂对香椿叶黄酮的吸附与解吸性能,并结合静态吸附动力学,筛选出适宜纯化香椿叶黄酮的大孔树脂为NKA-9。运用静态与动态吸附、解吸实验,研究得出NKA-9纯化香椿叶黄酮的最佳工艺条件为:选取70 m L 7 mg/m L的香椿叶提取物(含Na Cl浓度为3 mol/L),上样流速2 BV/h,用80 m L 60%乙醇溶液(p H 6)为洗脱剂,以2 BV/h的流速洗脱。在该条件下,香椿叶黄酮含量由纯化前81.272 3 mg/g增加到纯化后219.970 2 mg/g。高效液相色谱结果分析表明,芦丁、金丝桃苷、异槲皮苷、槲皮苷、阿福豆苷5种黄酮类单体物质含量均提高到纯化前的3倍以上。该工艺能有效地富集纯化香椿叶黄酮类物质,槲皮苷是此香椿叶黄酮类化合物的主要组分,含量是其他4种单体总量的2倍左右。

In order to find the most appropriate macroporous resin to purify total flavonoids from Toona sinensis leaf extract,the adsorption and desorption performance of nine kinds of macroporous resins for flavonoids from Toona sinensis leaves were investigated based on adsorption and desorption ratios,and kinetic studies of the static adsorption of flavonoids were also performed.NKA-9 resin was found to be suitable for the purification of total flavonoids from Toona sinensis leaves.By using dynamic adsorption and desorption experiments,the optimum purification conditions were obtained as follows：70 m L of 7 mg/m L Toona sinensis leaf extract（containing 3 mol/L Na Cl）was loaded onto the column at a flow rate of 2 BV/h and eluted with 80 m L of 60% ethanol（p H 6）at a flow rate of 2 BV/h.Under these conditions,the content of total flavonoids was increased from 81.272 3 to 219.970 2 mg/g.The results of high performance liquid chromatography（HPLC）analysis indicated that the contents of rutin,hyperoside,isoquercitrin,quercitrin and afzelinare were increased by more than 3 folds,respectively,compared with those before purification.The approach presented in this study enables effective enrichment of total flavonoids from Toona sinensis leaves.Quercitrin,which is two times more abundant than four other compounds,is the main flavonoid compound from Toona sinensis leaves.