石榴皮木醋液的化学成分及抗氧化活性

Chemical Constituents and Antioxidant Activities of Wood Vinegar from Pomegranate Husk

为探索石榴皮资源利用的新途径,以石榴皮为原料经干馏（350~500℃）制备了木醋液,并采用气相色谱-质谱联用仪分析其化学成分,通过对1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基和羟自由基清除实验评价其抗氧化活性。结果显示,石榴皮木醋液的总酚、总酮和有机酸含量分别为10.92、7.81 mg/m L和2.53%;共检测出27种有机化合物,包括酚类、有机酸、醇类、烷烃、醛类及酮类化合物等。石榴皮木醋液对DPPH自由基和羟自由基清除力的VC当量分别为10.22μg/mg和45.19μg/mg。结果表明,石榴皮木醋液可作为一种天然抗氧化剂资源,具有一定的开发利用价值。

In order to explore a new approach to the utilization of pomegranate husk,wood vinegar was prepared by pyrolyzing pomegranate husk in the temperature range of 150–400 ℃.Chemical constituents of the wood vinegar were analyzed by gas chromatography-mass spectrometry（GC-MS）.Its antioxidant activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl（DPPH）radical and hydroxyl radical scavenging methods.The results showed that the contents of total phenols,total ketones and organic acid in the wood vinegar were 10.92 mg/m L,7.81 mg/m L and 2.53% respectively.A total of 27 compounds were detected by GC-MS analysis from the wood vinegar,mainly including phenols,organic acids,alcohols,alkanes,aldehydes and ketones.Its scavenging activities against DPPH and OH hydroxyl radicals were 10.22 μg/mg and 45.19 μg/mg VC equivalent,respectively.This study suggested that wood vinegar from pomegranate husk has the potential to be used as a natural antioxidant resource.