摘要
利用纳米金/石墨烯复合纳米材料以及己二烯雌酚小分子修饰电极,研制了一种新型的超灵敏己二烯雌酚复合纳米电化学生物传感器;制备了己二烯雌酚抗原和多克隆抗体;并以K_3Fe(CN)_6为探针,利用己二烯雌酚抗体与半抗原之间的竞争反应实现了对己二烯雌酚的超灵敏电化学免疫检测。结果表明:纳米金/石墨烯复合纳米材料具有优异的增敏性和可修饰性。研制的复合纳米电化学生物传感器具有很高的检测灵敏度,己二烯雌酚质量浓度在1~6 000 ng/m L的范围有良好的线性关系,检测限可达到0.05 ng/m L;传感器修饰己二烯雌酚小分子具有简单、重复性和稳定性好的优点。对猪肉、鸭肉、牛肉以及奶粉实际样品进行了己二烯雌酚含量的分析,平均回收率在96.5%~103.7%之间,结果令人满意。
A novel electrochemical immunosensor was developed by modifying the electrode with gold nanoparticles/graphene composite material.The immunogen was prepared by conjugating dienestrol(hapten)to the carrier protein(bovine serum albumin)and used to generate polyclonal antibodies.Cyclic voltammetry and differential pulse voltammetry were employed to monitor the fabrication process of immunoreaction system in solutions containing the redox probe K_3Fe(CN)_6.The results revealed that gold nanoparticle/grapheme composites could effectively modify the electrode and enhance its sensitivity.The biosensor showed excellent detection performance with a broad linear range of 1–6 000 ng/m L and a supersensitive detection limit of 0.05 ng/m L.In addition,the biosensor modified with the small molecule of dienestrol had the advantages of simplicity,reproducibility and stability.Good recoveries in the range of 96.5%–103.7% were obtained in the detection of pork,dunk,beef and milk powder samples.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第8期201-206,共6页
Food Science
基金
江苏省科技厅科技支撑计划社会发展项目(BE2014720)
江苏省农业科技自主创新资金项目(CX(14)2127)