高效液相色谱法同时测定葡萄酒中16种添加剂

Simultaneous Determination of 16 Additives in Wine by High Performance Liquid Chromatography

研究同时测定葡萄酒中16种添加剂的高效液相色谱方法。该法主要采用CAPCELL PAK C_（18）柱（150 mm×4.6 mm,5μm）分离,以甲醇-20 mmol/L乙酸铵溶液为流动相,采取梯度洗脱,在波长250 nm处进行测定。结果表明,在0.1~20 mg/L的质量浓度范围内线性相关系数良好,相关系数范围为0.999 3~0.999 7,方法检出限范围为0.01~0.5 mg/L,相对标准偏差在0.3%~6.3%之间,平均回收率范围在76.5%~108.5%之间。本方法简便、可靠,能满足对市场上葡萄酒中相关非法添加剂的检测监控要求。

A high performance liquid chromatography（HPLC）method was developed in this study for the simultaneous determination of 16 additives in wine.Chromatographic separation was performed using gradient elution on CAPCELL PAK C18 column（150 mm × 4.6 mm,5 μm）with methanol-20 mmol/L ammonium acetate as mobile phase,followed by detection at 250 nm.The results showed that the method exhibited an excellent linearity in the concentration range of 0.1–20 mg/L with a correlation coefficient of 0.999 3–0.999 7 and had a good repeatability with relative standard deviation（RSD）of 0.3%–6.3%.The limits of detection（LOD）were between 0.01 and 0.5 mg/L,and average recoveries ranged from 76.5% to 108.5%.The present method is simple and accurate and can meet the requirements for the analysis of illicit additives in wines.