Ⅰ型胶原基因COL1A2多态性与动脉粥样硬化性脑梗死及动脉粥样硬化斑块稳定性的关系

Relationship between collagen Ⅰ gene COL1A2 polymorphisms and atherosclerotic cerebral infarction,stability of carotid arteriosclerotic plaque

目的探讨Ⅰ型胶原COL1A2基因rs42524位点多态性与动脉粥样硬化性脑梗死及颈动脉粥样硬化斑块稳定性的关系。方法用彩色多普勒超声诊断仪对289例动脉粥样硬化性脑梗死患者进行颈动脉超声检测,根据颈动脉粥样硬化斑块稳定性进行分组,同时以107名无颈动脉斑块的健康者作为对照组。运用PCR-限制性酶切片段长度多态性方法检测Ⅰ型胶原COL1A2基因rs42524位点多态性。对结果进行比较分析。结果脑梗死组与对照组COL1A2基因rs42524位点各等位基因频率及基因型分布的差异无统计学意义(均P>0.05)。根据颈动脉超声结果,脑梗死组患者分为稳定斑块亚组108例,易损斑块亚组181例。易损斑块组和稳定斑块组COL1A2基因rs42524位点各等位基因频率及基因型分布的差异无统计学意义(均P>0.05)。结论 COL1A2基因rs42524多态性与动脉粥样硬化性脑梗死及颈动脉硬化斑块稳定性均无关。

Objective To observe the relationship between rs42524 polymorphisms of collagen Ⅰ COL1A2 gene and atherosclerotic cerebral infarction,stability of carotid arteriosclerotic plaque. Methods Carotid ultrasound detection by Color Doppler ultrasonography was used to detect 289 patients with acute atherosclerotic cerebral infarction. These patients were divided into stable plaque and vulnerable plaque subgroups according to the carotid atherosclerotic plaque stability. Then 107 healthy individuals who had no carotid plaque were collected as the control group. Rs42524 polymorphism of COL1A2 gene was detected by PCR-restriction fragment length polymorphism. The results were analyzed. Results There were no significant differences of the frequencies of genotypes and alleles of rs42524 of COL1A2 gene between the cerebral infarction group and the control group（ all P〉0. 05）. According to the results of carotid ultrasound detection,the cerebral infarction group patients were divided into stable plaque subgroup including 108 cases,and vulnerable plaque subgroup including 181 cases. Rs42524 of COL1A2 gene showed no significant differences in the frequencies of alleles and genotypes between stable plaque subgroup and vulnerable plaque subgroup（ all P〉0. 05）. Conclusion Rs42524 polymorphism of COL1A2 gene is meaningless to the onset risk of atherosclerotic cerebral infarction and the stability of carotid atherosclerotic plaque.