膀胱癌组织中E-cd和cyclin D1的表达意义

Expression and clinical significance of E-cadherin and cyclin Dl in transitional cell carcinomatissue of bladder.

目的 研究上皮钙粘附素(E-cd)和细胞周期蛋白D1(cyclin D1)在膀胱移行细胞癌的表达水平及其相关,井探讨其与癌的发生,发展及生物学行为的关系。方法 应用免疫细化LSAB法检测69例膀胱癌和8例正常膀胱组织中E-cd和cyclin D1的表达。结果 膀胱癌组织中E-cd阳性表达率为50.7％(35/69)和cyclin D1阳性表达率为57.9％(40/69),与正常膀胱相比均有显著意义(P<0.05)。Ecd和cyclin D1阳性表达的病理分级分别为1级72.2％、33.3％,Ⅱ级65.2％、56.5％,Ⅲ级25％、71.4％,Ⅰ级与Ⅲ级均有显著意义(P<0.05)。复发组的阳性表达分别为29.7％、62.1％。生存时间大于5年的阳性表达分别为83.3％、39.2％。结论 E-cd的低表达或缺失与膀胱癌的侵袭、复发和预后密切相关,cyclin D1在膀胱癌形成中起重要的作用,二者均可以作为判断膀胱癌的病理分级、术后复发和临床预后的指标。

Objective To study the expression and clinical significance of E-cadlierin(E-cd) and cyclin Dl in transitional cell carcmoma(TCC) of bladder. Methods lmmunohistochemical method was used to detect the expression of F.-cd and cyclin Dl in 8 cases of normal bladder and 69 cases of TCC s of bladder. Results In 69 TCCa. 50.7% (35/69) was E-cd positive , 57.9% (40/69) was cyclin Dl positive; E-cd and Cyclin Dl were all negative in 8 normal bladder tissues ( p<0. 05) . In different pathological grades of TCCs, the positive rates of E-cd and cyclin Dl were 72. 2% , 33.3% in grade 1. , 65.2% , 56. 5% in grade Ⅱ , 25.0%, 71.4% in grade Ⅲ , respectively. There was a remarkably statistic significance between grade I and grade 3 (p<0.05). In recurrent TCCs the positive rates of E-cd and cyclin Dl were 29.7% and 62.1% respectively. The positive rate of E-cd was 83. 3% , and that of cyclin Dl was 39. 2% in those cases of living more than 5 years. Conclusion The low expression or deficiency of E-cd is closely related to the invasion,recurrence and prognosis of TCCs,and cyclin Dl is very important in pathogencsis of TCCs. Roth of them can be the indictors of pathological grades, recurrence and clinical prognosis.