脑心通胶囊提取物通过增强CXCR4表达促进心脏干细胞迁移

Naoxintong extract promotes migration of cardiac stem cells through enhancing expression of CXCR4

[目的]探讨脑心通(NXT)对小鼠心脏干细胞(CSCs)CXCR4表达的影响。[方法]应用实时荧光定量聚合酶链式反应(PCR)芯片技术研究脑心通对心脏干细胞功能基因表达的影响。采用流式细胞术检测脑心通对心脏干细胞CXCR4表达的影响。应用蛋白免疫印迹法(Western Blot)检测脑心通对心脏干细胞CXCR4蛋白表达的影响。应用Transwell法测定脑心通对SDF-1诱导的心脏干细胞迁移能力的影响。[结果]芯片结果发现脑心通促进心脏干细胞CXCR4基因的表达;流式细胞技术结果显示,脑心通促进心脏干细胞APC-CXCR4阳性细胞率;Western Blot结果表明,脑心通促进心脏干细胞CXCR4蛋白表达;Transwell迁移实验结果显示,脑心通促进SDF-1诱导的心脏干细胞的迁移作用。[结论]脑心通可以促进心脏干细胞CXCR4的表达。

[Objective] To study the effect of Naoxintong（NXT） on cardiacstem cell function and to explore its possible mechanism. [Methods] We use real-time RT-PCR array technology, Western-blot,flow cytometry and transwell methods to investigate the expression of CXCR4 on the cardiac stem/progenitor cell by NXT. [Results] The results showed that the expression of CXCR4 gene and CXCR4 protein in cardiac stem cell were both increased by NXT. [Conclusion] The migration of cardiac stem cell is promoted by NXT through the SDF-1/CXCR4 axis.