猪链球菌Lmb、Sao、ZnuA蛋白的抗原表位、二级结构分析及重组表位疫苗分子的设计

Antigen epitopes,secondary structure analysis for Lmb,Sao,ZnuA of Streptococcus suis and molecular designing of recombinant epitope vaccine

[目的]利用生物信息学方法设计猪链球菌候选疫苗蛋白Lmb、Sao、Znu A的重组表位多肽分子。[方法]通过ABCpred和Bepi Pred方案,预测猪链球菌Lmb、Sao、Znu A蛋白的B细胞表位。运用神经网络与量化矩阵法预测蛋白的CTL表位。使用MHC-Ⅱ类分子结合肽预测蛋白的Th表位。采用DNASTAR软件与SOPMA服务器预测蛋白二级结构,进而验证获得的B/T细胞表位的准确性。通过DNASTAR Protean软件重组拼接获得的B/T细胞抗原表位,设计抗原性较好的猪链球菌重组表位多肽。[结果]猪链球菌Lmb、Sao、Znu A蛋白的优势B细胞表位数分别为4个、4个和3个;CTL表位数各为1个;Th表位数分别为2个、1个和1个。二级结构预测显示这些表位大多处于蛋白易于产生表位的暴露表面、无规则卷曲与转角等位置。并设计获得抗原性较好的重组表位多肽。[结论]设计了抗原性较好的猪链球菌Lmb、Sao、Znu A蛋白的重组表位多肽。

[Objective]Using bioinformatics methods to design recombinant epitope peptides from vaccine candidate protein Lmb,Sao and Znu A of Streptococcus suis. [Methods]Synthesis of ABCpred and Bepi Pred methods predicted B cell epitopes for Lmb,Sao and Znu A of Streptococcus suis. CTL epitopes were predicted using neural network method; and MHC- Ⅱ molecule binding peptides program was used to predict Th cell epitopes. DNASTAR software and SOPMA were used to predict protein secondary structure which verified the accuracy for the B / T cell epitopes. Besides,Streptococcus suis epitopes peptide was designed with better immunogenicity by DNASTAR Protean software. [Results]Lmb,Sao,Znu A protein of Streptococcus suis B cell epitopes were 4,4 and 3,each protein had 1 CTL cell epitopes,which Th cell epitopes were 2,1 and 1. The results of secondary structure showed that most of the B / T cell epitopes were located in the exposed surface,the random coil and the corner. The recombinant epitope peptides with good immunogenicity were designed. [Conclusion]A recombinant epitopes peptide was designed for Lmb,Sao,Znu A protein of Streptococcus suis.