面部表情疼痛量表和行为学评估慢性胰腺炎大鼠的疼痛

Assessment of pain in rats with chronic pancreatitis using behaviour and the rat grimace scale

目的研究大鼠面部表情疼痛量表检测慢性胰腺炎大鼠的疼痛可行性,为疼痛治疗提供新的观察方法。方法成年雄性Wister大鼠28只,随机分为两组,每组14只。实验组一次性经尾静脉注射二丁基二氯化锡溶液,对照组注射等剂量的乙醇和甘油,测量注药前后大鼠体质量和机械痛行为发生的频率,观察大鼠面部表情。2周后处死大鼠,HE染色观察大鼠胰腺组织病理变化。结果与对照组比较,实验组大鼠胰腺组织有明显慢性炎症表现,体质量明显降低(P<0.01),腹部机械痛超敏反应明显增加(P<0.01),面部疼痛表情加重。结论可以用面部表情疼痛量表评估慢性胰腺炎大鼠的疼痛,有助于实现基础科学向临床应用转化。

Objective To investigate whether rat grimace scale（RGS）could be used to assess pain in chronic pancreatitis,so as to provide evidence for pain research and clinical assessment of rat pain.Methods Twenty-eight adult male wister rats were evenly randomized into two groups（n=14）：an experimental group and a control group.The experimental group was intravenously given 8mg/kg body weight dibutyltin dichloride（DBTC）to induce chronic pancreatitis,and the control group was injected with ethanol and glycerin solution.Abdominal hypersensitivity,RGS scores and weight at different time points was detected.HE staining was used to detect the histological changes of pancreatic tissue.Results Compared with the control group,the rats in the experimental group showed chronic inflammation in pancreatic tissue in two weeks.There was a significant increase in the number of abdominal withdrawals（P〈0.001）and RGS in the experimental group.Conclusion Rat grimace scale might lead to a successful transition of basic science findings into clinical application.