白细胞介素-22及白细胞介素-22结合蛋白表达与乳腺癌发生发展的关系

Expression and significance of interleukin -22 and interleukin -22 binding protein in patients with breast cancer

目的观察乳腺癌患者外周血及组织中白细胞介素（IL）-22及IL-22结合蛋白（IL-22BP）表达水平对乳腺癌发生发展的影响。方法采用酶联免疫吸附试验法（ELISA）检测95例乳腺癌患者和20例乳腺良性肿瘤患者血清IL-22和IL-22BP表达水平，用免疫组织化学（IHC）方法检测两者存乳腺癌组织和癌旁组织表达。结果乳腺癌患者外周血IL-22为（64．5±22．1）pg／ml，显著高于乳腺良性肿瘤患者外周血IL-22水平[（23．7±11．4）pg／ml，P〈0．01]；乳腺癌患者外周血IL-22BP为（260．6±70．9）pg/ml，显著低于乳腺良性肿瘤患者外周血IL-22BP水平[（472．1±156．3）pg／ml，P〈0．01]。IL-22在乳腺癌组织阳性表达率为71．57％，在癌旁组织阳性表达率为24．21％，两者差异有统计学意义（x^2=42．71，P〈0．01）。IL-22BP在乳腺癌组织中的阳性表达率为27．37％，在癌旁组织中的阳性表达率为74．74％，两者差异有统计学意义（x^2=42．65，P〈0．01）。11．-22在细胞组织学Ⅲ级、有淋巴结转移、肿瘤最大径〉2cm、临床分期Ⅲ期、人类表皮生长因子受体-2（Her-2）过表达和细胞核增殖抗原（Ki-67）阳性率≥14％组明显升高（P〈0．01或P〈0．05）。而IL-22BP在上述组中明显降低（P〈0．01或P〈0．05）。结论IL-22的高表达或其拮抗蛋白IL-22BP的低表达，与乳腺癌的发生、发展及转移关系密切。

Objective To investigate the expression of interleukin （IL） -22 and IL -22 binding protein （ IL - 22BP） in patients with breast tumor and to analyze the relationship between them and patho- logical characteristics. Methods Ninety -five patients with breast cancer, and 20 patients with breast be- nign tumor were included. The concentrations of IL- 22 and IL -22BP in serum were measured by enzyme - linked immunosorbent assay （ELISA）. Their expression in breast cancer tissues and non - can- cerous adjacent breast tissues were examined by immunohistochemistry. Statistical analysis of the relevance of IL - 22, IL - 22BP and clinicopathological variables was performed. Results The level of IL - 22 in pe- ripheral blood of patients with breast cancer was （64. 5 ± 22. 1 ） pg/ml, significantly higher than that in be- nign tumor [ （23.7±11.4） pg/ml] （P 〈 0. 01 ）. The level of IL- 22BP in peripheral blood of patients with breast cancer was （260. 6 ± 70. 9 ） pg/ml, significantly lower than that in benign tumor [ （472. 1 ± 156. 3） pg/ml] （P 〈 0. 01 ）. The expression rate of IL -22 was 71.57% in breast cancer tissues and 24.21% in non- cancerous adjacent breast tissues respectively （P〈 0. 01 ）. The expression rate of IL-22BP was 27.37% in breast cancer tissues, and 74. 74% in non -cancerous adjacent breast tissues （ P 〈 0. 01 ）. The expression of IL -22 and IL -22BP was significantly positively correlated with histologi- cal grade, axillary lymph nodes metastasis, larger tumor size, tumor stage, proliferation cell nuclear anti- gen （Ki-67） ≥ 14% and human epidermal growth factor rceeptor- 2 （Her- 2） positive status （P 〈 0. 01 or P 〈0. 05） , but not with age at diagnosis, menopausal status, estrogen receptor （ER） status and progesterone receptor （PR） status. Conclusion The high expression of IL -22 and low expression of 11,- 22BP in patients with breast cancer might be closely related with the pathogenesis and development of breast cancer.