中性粒细胞明胶酶相关载脂蛋白在非小细胞肺癌组织的表达

Expression of neutrophil gelatinase-associated lipocalin in non-small cell lung cancer tissues

目的 探讨中性粒细胞明胶酶相关载脂蛋白（NGAL）在非小细胞肺癌（NSCLC）组织中的表达及意义.方法 以130例NSCLC患者切除肿块标本作为实验组,肿块边缘5 cm以上的远癌组织作为对照组,其中腺癌60例,鳞癌70例.通过实时荧光定量聚合酶链反应（FQ-PCR）的方法检测NSCLC组织中NGAL mRNA的表达;免疫组织化学技术检测NGAL蛋白的表达,并分析NGAL蛋白的表达与患者临床特征的关系.结果 （1）腺癌组织NGAL mRNA的FQ-PCR结果与β-肌动蛋白（β-actin）的ACt值之差（-2.9±1.4）,与鳞癌组织（-2.4±1.1）比较,差异无统计学意义（P＞0.05）;腺癌组织与鳞癌组织较癌旁组织（-1.5±1.4）比较,差异均有统计学意义（P＜0.05）.（2）NSCLC中,鳞癌组织中NGAL蛋白阳性表达率为55.71％ （39/70）,腺癌组织阳性表达率为78.33％（47/60）,远癌组织为31.53％ （41/130）,三者阳性表达率差异有统计学意义（x^2=5.942,P＜0.05）.（3） NSCLC组织中NAGL蛋白的表达和患者年龄、性别、是否吸烟及有无淋巴结转移无相关（P＞0.05）.结论 NGAL在NSCLC组织内呈高表达,并与NSCLC分型相关,可能成为早期诊断NSCLC的一个标志物.

Objective To investigate the neutrophil gelatinase-associated lipocalin （NGAL） expression and clinical significance in non-small cell lung cancer （NSCLC） tissues.Methods The surgical treatment of non-small cell lung cancer patients resected tumor specimens of 130 cases of the experimental group,the distance of the cut edge of tumor more than 5cm much cancerous tissue as a control group,60 cases of adenocarcinoma,squamous cell carcinoma and 70 cases.By real-time fluorescent quantitative polymerase chain reaction （FQ-PCR） method to detect NGAL mRNA expression in NSCLC tissues;the protein was detected by immunohistochemistry NSCLC tissues NGAL,NGAL expression and analysis and clinical characteristics of patients with protein relationship.Results （1） Adenocarcinoma NGAL mRNA real-time fluorescence quantitative results and ACt difference of β-actin （-2.9 ± 1.4）,and squamous cell carcinoma （-2.4 ± 1.1） compared to no significant difference （P 〉 0.05）;Adjacent tissues （-1.5 ± 1.4） compared with adenocarcinoma and squamous cell carcinomas （P 〈 0.05）.（2） Non-small cell lung cancer,squamous cell carcinoma NGAL protein positive rate 55.71% （39/70）,adenocarcinoma positive rate was 78.33% （47/60）,adjacent tissues was 31.53% （41/130）,three positive expression rate difference was statistically significant （x^2 =5.942,P 〈 0.05）.（3） Non-small cell lung cancer NAGL protein expression and patient＇ s age,sex,smoking and lymph node metastasis was no significant correlation （P 〉 0.05）.Conclusion NGAL in the non-small cell lung cancer was highly expressed,and the classification of non-small cell lung cancer-related,may be an early diagnosis of non-small cell lung cancer a marker.