丙烯腈对雄性小鼠生殖细胞作用的研究

Studies on the effects of acrylonitrile on germ cells in male mice

目的 探讨丙烯腈对雄性小鼠生殖细胞的毒性作用。方法 将 40只昆明种雄性小鼠随机分成 5组 ,阴性对照组 (生理盐水 0 1ml/ 10g)、低剂量组 (丙烯腈 2mg/kg)、中剂量组 (丙烯腈 4mg/kg)、高剂量组 (丙烯腈 8mg/kg)和阳性对照组 (环磷酰胺 2 0mg/kg)。每天皮下注射 1次 ,连续 5天。实验结束处死动物 ,取材 ,做初级精母细胞染色体畸变分析及精子畸形试验。结果 皮下注射丙烯腈 2mg/kg ,4mg/kg和 8mg/kg组小鼠睾丸初级精母细胞染色体畸变率、细胞畸变率及精子畸形率均显著高于阴性对照组。结论 丙烯腈对雄性小鼠的生殖细胞有致突变效应 ,并对精子的生成有毒害作用。

Objective To study the effects of acrylonitrile(AN)on germ cells in male mice.Methods Forty male mice of Kunming strain were divided randomly into five groups,i.e.,negative control group treated with normal saline 0 1ml/10g of body weight,low dose group with 2 mg/kg of AN,medium dose group with 4mg/kg of AN,high dose group with 8 mg/kg of AN and positive control group with 20 mg/kg of cytoxan by subcutaneous injection once daily for five days.Then,the animals were killed and their bio specimens were collected and the chromosome aberration of primary spermatocyte and sperm deformity test were performed for them.Results The rates of chromosome aberration of primary spermatocyte,cell deformity and sperm deformity were significantly higher in the animals treated with 2 mg/kg,4 mg/kg and 8 mg/kg of AN by subcutaneous injection than those with negative controls.Conclusions Acrylonitrile could have mutagenic effects in male mice,which was hazardous and toxic to their spermatogenesis.