LEF1在卵巢上皮癌中的表达及意义

Expression and significance of LEF1 in epithelial ovarian carcinoma

目的研究淋巴增强因子1(LEF1)在卵巢上皮癌肿瘤组织中的表达情况,分析其表达与临床病理特征之间的关系。方法收集手术切除的卵巢上皮肿瘤新鲜标本:卵巢良性上皮肿瘤24例,交界性上皮肿瘤17例,恶性上皮肿瘤35例;另选取因子宫肌腺瘤、乳腺癌、宫颈癌疾病切除的正常卵巢组织13例作为对照。提取组织蛋白,采用Western blot检测不同组织LEF1的表达情况,分析恶性上皮肿瘤组织中LEF1的表达与患者年龄、组织学类型、分化程度等临床病理特征之间的关系。结果与子宫良性疾病、良性、交界性卵巢上皮肿瘤相比,恶性上皮肿瘤中LEF1的表达显著增高(P<0.05),良性及交界性肿瘤与对照组相比无统计学差异(P>0.05)。在卵巢恶性上皮肿瘤中,LEF1在子宫内膜样腺癌组织中的表达高于浆液性癌及黏液性癌(P<0.05),浆液性癌与黏液性癌组织中LEF1的表达无显著差异(P>0.05);在有淋巴结转移病例肿瘤组织中的表达高于无淋巴结转移病例(P<0.05);在FIGO分期Ⅲ期+Ⅳ期病例肿瘤组织中的表达高于Ⅰ期+Ⅱ期病例(P<0.05);LEF1在不同年龄及不同分化程度肿瘤组织中的表达无显著差异(P>0.05)。与原发灶相比,LEF1在转移性淋巴结组织中的表达显著增高,差异有统计学意义(P<0.05)。结论 LEF1在卵巢上皮癌组织中表达上调,与组织类型、淋巴结转移及临床分期相关,可能参与卵巢癌侵袭、转移的病理过程。

Objective To explore the expression and significance of lymphoid enhancer factor-1（ LEF1） in tissues of human epithelial ovarian carcinoma. Methods The expression of LEF1 in 24 cases of benign ovarian epithelial tumor tissues,17 cases of borderline epithelial tumor tissues,35 cases of malignant epithelial tumor tissues and 13 control tissues was detected by Western blot. The correlation between LEF1 and clinicopathological data was analyzed in malignant epithelial tumor patients. Results The expression of LEF1 in malignant epithelial tumors was significantly higher than that in control tissues and benign ovarian epithelial tumor tissues and borderline ovarian tumor tissues（ P〈0. 05）,but there was no statistical difference between control tissues and benign ovarian epithelial tumor tissues,borderline ovarian tumor tissues（ P〉0. 05）. LEF1 expression in endometrial adenocarcinoma was higher than that in serous and mucinous carcinoma（ P〈0. 05）,higher in lymph node metastasis tumor tissues than that in non-lymph node metastasis tissues（ P〈0. 05）,higher in FIGO stage Ⅲ ＋ Ⅳ cases than that of stageⅠ＋Ⅱ（ P〈0. 05）,but the expression of LEF1 in different age and different degree of differentiation of tumor tissues showed no significant difference（ P〉0. 05）. Compared with the primary tumor,LEF1 expression in metastatic lymph node tissue was significantly increased（ P〈0. 05）. Conclusion LEF1 is up-regulated in malignant ovarian epithelial tumor,and related with histological type,lymph node metastasis and clinical stage,which indicates that it may be involved in the pathological processes of invasion and metastasis.