摘要
目的:探究糖尿病大鼠Nfic的表达状况及其与其他成骨相关基因表达改变。方法:6周龄Wistar雄性大鼠30只,随机分为对照组和糖尿病模型组。模型组大鼠腹腔注射链脲佐菌素(65mg/kg)建立1型糖尿病模型。观察两组大鼠体重、血糖变化;模型建成后8周处死大鼠,Q-PCR法检测双侧股骨Nfic、Runx2、OSX(Oster ix)、COL2A1(Ⅰ型胶原)、OC(骨钙素)、ALP、BMP-2、IGF-1(胰岛素样生长因子)的mRNA表达水平。结果:8周后,模型组大鼠体重降低或保持不变,对照组大鼠体重持续稳定增长;模型组血糖持续保持在高血糖水平,对照组血糖位于正常范围;Q-PCR检测发现,Nfic、Runx2、OSX、COL2A1、OC、IGF-1、BMP-2基因mR NA表达中,模型组明显低于对照组。ALP基因的mRNA表达中,模型组显著高于对照组(P<0.01)。结论:1型糖尿病大鼠的高糖状态使得Nfic等成骨相关基因表达下降,成骨标记产物降低。
Objective: To investigate the expression of Nfic gene and its correlation with other osteogenesis related genes in diabetic rats. Methods: 30 male Wistar rats(6-week-old) were randomly divided into 2 groups, including experiment [B;n=20; 65 mg/kg of STZ, single i.p.] group and control [A; n=10;citrate buffer solution i.p.] group. Overt behavior, body weights and levels of blood glucose were evaluated at the 0th, 2nd, 4th, 6th, 8th week respectively. All rats were executed at the 8th week to collect the samples of femur. Q-PCR method was used for detecting Nfic, Runx2, OSX, COL2A1, OC,ALP, BMP-2, IGF-1 m RNA expression level. Results: 8 weeks later, the weight of model group rats declined or remained unchanged, the weight of control group rats were had a steady growth; Q-PCR showed that, compared with control group,Nfic, Runx2, OSX, COL2A1, OC, IGF-1, BMP-2m RNA declined significantly, meanwhile, ALP m RNA increased.Conclusion: The expression of Nfic and other osteogenesis related genes decreased in the type 1 diabetic rats, at the same time, the osteogenesis markers product reduced.
出处
《中华老年口腔医学杂志》
2016年第2期72-76,共5页
Chinese Journal of Geriatric Dentistry
基金
企事业单位合作课题(项目编号:HXKTLIYANAN001)