特异性免疫应答在肝郁证与脾虚证及相互关系的初步研究

Experimental Observation of the Specific Immune Response Basis About the Relationship Between the Liver-qi Stagnation Syndrome And the Spleen Deficiency Syndrome

目的 探讨特异性免疫应答在肝郁证与脾虚证相互关系研究中的初步研究。方法 以肝郁证与脾虚证模型大鼠为对象 ,给予柴胡疏肝散和四君子汤进行对应治疗与交叉治疗 ,采用淋巴细胞转化试验 (LTT)和免疫比浊法分别观察T、B淋巴细胞活性和血清IgG、IgM等特异性免疫应答指标的变化。结果 各组间IgG的水平未显示明显变化 (P >0 0 5 )。肝郁证与脾虚证IgM的水平均降低 ,但肝郁证降低得更为明显 (P <0 0 5 ;0 0 1) ;柴胡疏肝散对IgM的水平没有影响 (P >0 0 5 ) ,四君子汤对肝郁证与脾虚证IgM的水平均有一定的升高作用 (P<0 0 5 )。肝郁证T细胞转化率显著升高 ,而柴胡疏肝散可使之恢复正常 (P <0 0 5 ) ,四君子汤对其无明显影响 (P >0 0 5 ) ;脾虚证T细胞转化率显著降低 ,四君子汤可使之恢复正常 (P <0 0 5 ) ,而柴胡疏肝散对其则无明显影响 (P >0 0 5 )。肝郁证B细胞转化率也表现为升高的趋势 ,柴胡疏肝散和四君子汤均能使之恢复正常 (P <0 0 5 )。结论 肝郁证与脾虚证的相关很可能发生在抗原提呈阶段 ,尤其是在TH 细胞接受抗原刺激引起活化这一过程 ,柴胡疏肝散通过逆转肝郁证T、B细胞活性增强、四君子汤通过逆转脾虚证T细胞活性增强而发挥作用。

Objective To survey the significance of specific immune response in the relationship between Sagnation the Liver-qi syndrome and the spleen Deficiency syndrome.Methods The rats with the Liver-qi Stagnation syndrome and the Spleen Deficiency syndrome were respectively corresponding and crossely treated with Radix Bupleuric dispersing the stagnated Liver-qi powder Decolion of Four Noble Drugs. Their T, B lymphocyte activity and specific immune response indexes changes (such as IgG, IgM in the serum) were respectively observed by lymphocyte transformation test and immunoturbidimetry. Results There were no notable changes about the IgG level among all kinds of groups. The IgM levels in the Liver-qi Stagnation syndrome and the Spleen Deficiency syndrome decreased, especially in the Liver-qi Stagnation syndrome (P<0 05; 0 01); Radix Bupleuric dispersing the stagnated Liver-qi powder had no striking effect on the IgM level (P>0 05), Decolion of Four Noble Drugs could increase the IgM level of the patients with the Liver-qi Stagnation syndrome and the Spleen Deficiency syndrome (P<0 05); The T lymphocyte transformation rate of the Liver-qi Stagnation syndrome obviously increased and Radix Bupleuric dispersing the stagnated Liver-qi powder could recover it to the normal level (P<0 05), but Decolion of Four Noble Drugs had no striking effect on it (P>0 05); The T lymphocyte transformation rate of the Spleen Deficiency syndrome obviously decreased, and Decolion of Four Noble Drugs could recover it to the normal level (P<0 05), but Radix Bupleuric dispersing the stagnated Liver-qi powder had no striking effect on it (P>0 05). The rate of B lymphocyte transformation the Liver-qi Stagnation syndrome's also showed the increasing tenderly (P<0 05); Both of Radix Bupleuric dispersing the stagnated Liver-qi powder and Decolion of Four Noble Drugs could recover it to the normal level (P>0 05). Conclusions The relationship between the Liver-qi Stagnation syndrome and the Spleen Deficiency syndrome could occur in the phase of antigen presentation, especially in the phase of the T H cell activation stimulated by antigen. Radix Bupleuric dispersing the stagnated Liver-qi powder could enhance T, B lymphocyte activities by reverse the Liver-qi Stagnation syndrome,and Decolion of Four Noble Drugs could enhance T lymphocyte by improving the Spleen Deficiency syndrome.