经混合醇改性后的戊二醛鞣制的牛心包免疫原性测定

Immunogenicity of Glutaraldehyde Tanned Bovine Pericardium with Polydiol-post Treatment

目的 本实验采用免疫细胞化学方法—Biotin -Streptavidin—Peroxidase(S .P法 ) ,在体外 ,对戊二醛鞣制和混合醇改性后的牛心包进行免疫原性检测。方法 取新鲜牛心包制备抗牛心包抗体 ,采用S .P法在体外进行免疫原性检测。结果 新鲜牛心包表现为强抗原性 ,戊二醛组和混合醇改性组的牛心包 ,抗原性显著降低 ,与对照组明显差异。结论 ⑴戊二醛鞣制的牛心包经混合醇改性处理后 ,免疫原性显著降低。⑵S .P法做为一种体外免疫检测方法 ,具有临床实用价值。

Objective In this comparative study, we used a new immunocellular chemistry technique,Biotin- Streptavidin-Peroxidase(S.P) method to test the immunologic response to glutaraldehyde-pretreatment or polydiol-post treatment bovine pericardium in vitro.Method S. P was used to measure the immunologic responses to three group of bovine pericardium.Results The results of S. P in fresch bovine pericardium group showed strongly iummunogenicity in vitro. Glutaraldehyde-pretreated or polydiol-post treated bovine pericardium showed less immunogenicity in vitro.Conclusions ⑴The immunogenicity of G-tBP with polydiol post-treated was reduced significantly.⑵ S.P is a new technique and sensitive method for testing the immunogenicity of bioprosthesis in vitro.