雷帕霉素对肝脏缺血-再灌注中线粒体DNA D-Loop区的修复作用

Recovery effect of rapamycin to mitochondrial DNA D-Loop in liver iachemia

目的研究大鼠肝脏缺血-再灌注(HIRI)损伤中mtDNA的损伤机制及雷帕霉素对其的修复作用。方法 SD大鼠60只,制作大鼠肝脏缺血-再灌注模型,分为A、B、C、D四组;A组:肝脏缺血30min处理;B组:缺血50min处理;C组:缺血50min并雷帕霉素干预处理;D组:假手术组。分别于术后24h、3d、5d处死,取部分肝组织进行线粒体DNA D-Loop区进行PCR扩增、测序,与GENBANK参考序列对比查找变异位点。结果在HIRI中存在DNA D-Loop区突变,其中C组大鼠mtDNA D-Loop区突变率低于B组(P<0.05)。结论 HIRI中mtDNA D-Loop区产生突变,而雷帕霉素可能通过降低线粒体DNA D-Loop区突变率而减轻其氧化损伤,从而对肝脏缺血-再灌注损伤过程产生保护作用。

Objective To evaluate the hepatic ischemia-reperfusion injury in rats of mitochondrial DNA damage and rapamycin to its repair effect.Methods To be established the whole liver ischemia-reperfusion injury in rats model,and divided into four groups which were group A（ischemia 30 min group）,Group B（ischemia 50 min group）,Group C（preoperative rapamycin lavage,50 min of ischemia group）,and Group D（control group）.24 hafter surgery,3,5days respectively execution groups of animals,take part in liver mitochondrial DNA D kit instructionsLoop area for amplification and product sequencing,compared with GENBANK reference sequence search mutation loci.Results Group C mitochondrial DNA D-Loop area mutation rate was lower than that in group B（P 0.05）.Conclusion Liver ischemia-reperfusion injury of mutations in mitochondrial DNA D-Loop area,rapamycin could alleviate the liver ischemia0 reperfusion injury,its mechanism may be through reduced mitochondrial DNA D-Loop area mutation rate so as to reduce the oxidative damage,which produces the process of liver ischemia-reperfusion injury protection.