Gli-1蛋白在食管鳞癌组织的表达及其临床意义

The expression and clinical significance of gli-1 protein in esophageal squamous cell carcinoma

目的检测食管鳞癌及其癌旁组织和食管正常组织的中Gli-1的表达水平,并探究其与食管鳞癌的临床及病理因素之间的关系。方法免疫组化法测36例食管癌及其癌旁组织和9例食管正常组织Gli-1表达水平。结果 Gli-1在食管鳞癌阳性表达率为55.6%;癌旁为13.9%;食管正常组织中为11.1%;组间差异有统计学意义（P〈0.05）;Gli-1在癌旁和正常组织中均显著低于鳞癌（P〈0.05）;Gli-1在癌旁和正常食管中表达差异无统计学意义（P〉0.0 5）。其阳性表达率在Ⅰ～Ⅱ期远低于Ⅲ～Ⅳ期（P〈0.05）,T1～T2远低于T3～T4（P〈0.05）,无淋巴转移的远低于转移（P〈0.05）。但其表达与年龄、性别、肿瘤位置、直径及分化程度无关（P〉0.05）。结论 Gli-1参与了食管鳞癌的发展进程,高表达的Gli-1可能是促进食管鳞癌浸润转移的因素之一。

Objective： Objective To observe the expression of Gli- 1 in esophageal squamous cell carcinoma（ ESCC）,tumor- adjacent tissues and normal tissues,and to investigate the expression and clinical significance of Gli- 1 protein in esophageal squamous cell carcinoma. Methods： The expression of Gli- 1 protein was detected by immunohistochemistry in36 cases ESCC,tumor- adjacent tissues and 9 cases normal esophageal tissues. Results： In esophageal squamous cell carcinoma,tumor- adjacent tissues and normal tissues,the positive expression rate of Gli- 1 were 55. 6%,13. 9%,and11. 1% respectively,there was significant difference between group（ P〈 0. 05）. The expression of Gli- 1 protein in tumor- adjacent and normal tissues was lower than that in esophageal squamous cell carcinoma. The difference was statistically significant（ P〈 0. 05）. No difference was found between tumor- adjacent and normal tissues about the expression of Gli- 1 protein（ P〈 0. 05）. The expression of Gli- 1was not only much lower in T1 ～ T2 than that in T3 ～ T4（ P〈 0. 05）but also much lower in clinical stage Ⅰ ～ Ⅱ than that in Ⅲ ～ Ⅳ（ P〈 0. 05）. The expression of Gli- 1 was higher in nodal metastases group than that in group without nodal metastases（ P〈 0. 05）. No correlations were found between Gli-1 protein and age,gender,tumor location,Tumor diameter,the degree of differentiation（ P〉 0. 05）. Conclusions： Up-regulation of Gli- 1 might be involved in the development of esophageal squamous cell carcinoma,and it might play a promoting role in the invasion and metastasis of esophageal squamous cell carcinoma.