碱蓬SgBADH的克隆与分析及植物表达载体构建

Cloning and Analysis of Sg BADH in Suaeda glauca and Construction of Its Plant Expression Vector

为研究碱蓬甜菜碱醛脱氢酶基因的功能,采用同源克隆方法,从碱蓬(Suaeda glauca(Bunge)Bunge.)中克隆到BADH全长c DNA,命名为Sg BADH,并对其序列结构和表达特性进行分析。生物信息学分析发现,Sg BADH编码500个氨基酸的亲水性蛋白,预测亚细胞定位于叶绿体。基因表达分析发现Sg BADH基因受Na Cl和ABA诱导表达,预示Sg BADH基因可能在碱蓬对盐胁迫的反应中起重要作用。本研究同时构建了Sg BADH植物表达载体并转化EHA105农杆菌,为进一步研究BADH基因的功能和碱蓬的耐盐分子机制奠定了基础。

In order to study the function of betaine aldehyde dehydrogenase gene from Suaeda glauca,the full-length c DNA of BADH gene,which was named as Sg BADH was cloned from Suaeda glauca by homologous clone strategy. The Sg BADH gene was analyzed through the bioinformatic method and expression pattern. Bioinformatic analysis showed that Sg BADH encoded a 500 amino acids hydrophilic protein,which was predicted to be located in chloroplast. Further analysis showed that,Sg BADH gene was induced by Na Cl and abscisic acid（ ABA） treatments,suggesting that it may play an important role in salt tolerance of S. glauca. At the same time,Sg BADH gene was inserted into a plant expression vector and transformed into Agrobacterium tumefacions strain EHA105,which laid a foundation for further studying the BADH function and molecular mechanism involved in salt resistance of S. glauca.