砀山酥梨细胞色素P450的基因组学分析

Genomics Analysis of Cytochrome P450 Monooxygenase Genes in Pyrus bretschneideri Rehd.

细胞色素P450在植物的苯丙烷类、生物碱和萜类等生物合成中起着非常重要的作用。为了更好地了解砀山酥梨中P450的种类和数量,利用砀山酥梨基因组的氨基酸和c DNA数据库对P450基因进行筛选,分析砀山酥梨全基因组中P450基因的种类、进化关系、基因的物理定位、以及二级结构元件。结果显示,在砀山酥梨基因组中发现并初步确定了226个P450基因,通过基因的定位分析确定了226个基因分布在17条染色体上,其中除了4、5、9号染色体上无基因簇分布外,其他的染色体都有基因簇的分布。通过基因结构和进化树之间的比较,进一步确定了基因结构和进化之间的相互联系。二级结构的分析表明,砀山酥梨P450蛋白序列同样具有P450蛋白特征性结构域。本研究结果为砀山酥梨P450基因功能的深入分析奠定了基础。

Cytochrome P450 plays a very important role in the biosynthesis of plant phenylpropanes,alkaloids and terpenoids,etc. In order to learn more about the type and quantity of P450 in Pyrus bretschneideri Rehd,P450 genome has been screened and analyzed based on the amino acids and c DNA database of Pyrus bretschneideri Rehd. The evolutionary relationships,gene physical mapping,as well as secondary structural elements of P450 in whole-genome of Pyrus bretschneideri Rehd are analyzed. A total of 226 P450 genes are initially identified in Pyrus bretschneideri Rehd genome. The distribution of 226 genes on the chromosomes can be known by gene mapping analysis. Many chromosomes contain gene clusters except the chromosome 4,5,9. The relationship of gene structure and evolution are further identified according to the comparison of the gene structure and evolutionary tree. Analysis of the secondary structure showed the P450 protein sequence of Pyrus bretschneideri Rehd also has the characteristic domain of P450 protein.These findings establishes the foundation for further analysis of the function of P450 gene in Pyrus bretschneideri Rehd.