酶解处理对小鼠模型中虾主要致敏蛋白的致敏性影响分析

Allergenicity Analysis of Enzymolysis on Shrimp Allergenic Proteins in Mouse Allergy Model

为探究酶解处理对动物模型中虾主要致敏蛋白的致敏性影响,68只雌性BALB/c小鼠随机分为生理盐水对照组、虾致敏蛋白组、酶解虾致敏蛋白组、酶对照组,腹腔注射各组溶液,测定小鼠的脾指数,血清中s Ig G、s Ig E、组胺含量,并观察灌胃处理后的小鼠小肠切片,探究酶解后蛋白的抗原性变化。结果表明,酶解处理使虾中主要致敏蛋白的α-螺旋和β-折叠比例分别下降3.5%和1.5%,β-转角跟无规则卷曲比例分别上升4.3%和1.7%,这些变化使小鼠体内s Ig E水平降低了40.44%。但虾致敏蛋白组的s Ig G和脾指数与酶解蛋白组相比差异不显著,且酶解蛋白组肠切片显示其炎症反应较致敏蛋白组更为明显。综上所述,酶解处理减弱了虾致敏蛋白的抗原性,在一定程度上降低了其致敏性,但是经过多次免疫,酶解后的致敏蛋白在小鼠体内依然能引起很强的过敏反应。本研究为探索致敏蛋白降敏机理提供了技术支撑。

To explore the effect of enzymolysis on shrimp allergenic proteins,68 female BALB / c mice were divided into four groups： blank control,shrimp allergenic proteins,shrimp allergenic protein hydrolysates and enzyme control.By intraperitoneal injection,the concentrations of s Ig G,s Ig E and histamine in serum were measured by ELISA,the spleen indexes and allergic symptoms of mice were observed.The results showed that： enzymolysis made the α- helix of shrimp allergenic proteins decreased by 3.5%,the β- sheet decreased by 1.5%,the β- turn and random increased by 4.3%and 1.7%.These changes made the level of s Ig E in mice were reduced by 40.44%.But the s Ig G and spleen between shrimp allergenic proteins group and protein hydrolysates group had no significant difference.And the protein hydrolysates group paraffin sections of intestine showed that the inflammatory response was more obvious than that of the shrimp allergenic proteins group.Conclusion： enzymolysis on shrimp allergenic proteins can reduce antigenicity to a certain extent,which reduces the allergenicity.But after several immune processes,protein hydrolysates still has very strong allergenicity.This research lays the groundwork for allergenic protein allergenicity reduction mechanism