利多卡因及MEGX在蛛网膜下腔麻醉致死犬体内死后再分布

The postmortem redistribution of the different dose of lidocaine and MEGX in dogs after subarachnoid administration

目的研究利多卡因及其代谢产物单乙基甘氨酰二甲苯胺(MEGX)在蛛网膜下腔麻醉致死犬体内的死后再分布规律。方法犬6只随机分为A、B两组,分别经蛛网膜下腔注射0.5倍(6.34mg/kg)和5倍(63.35mg/kg)硬膜外麻醉极量的盐酸利多卡因,于死后0h、12h、24h、36h、72h取心血、外周血、肝、脑等,采用高效液相色谱法(HPLC)检测其中利多卡因及MEGX的含量。结果 A组犬死后72h,心血、外周血和脑中利多卡因含量与死亡当时的比值(Ct/C0)分别为4.74,14.87,7.67,均呈上升趋势(P<0.05),MEGX含量与死亡当时含量差异无统计学意义(P>0.05);B组犬死后72h,心血中利多卡因含量Ct/C0值为0.36,呈下降趋势(P<0.05),脑中为3.48(P<0.05)呈升高的趋势,肝中MEGX含量与死亡当时相比差异无统计学意义(P>0.05)。结论蛛网膜下腔不同剂量麻醉致死犬体内利多卡因均会发生死后再分布,MEGX未发生死后再分布。

Objective To study the postmortem redistribution of lidocaine and its metabolite monoethylglycinexylidide（ MEGX） in dogs after subarachnoid administration. Methods 6 mongrel dogs were randomly divided into A and B groups. The dogs were given the subarachnoid administration of lidocaine hydrochloride respectively with the dose of 0. 5 times（ 6. 34 mg / kg） and 5 times（ 63. 35 mg / kg）,the cardiac blood,peripheral blood,liver,brain of the dog cadavers were taken out to detect the content of lidocaine and MEGX at different time after death（ 0h,12 h,24h,36 h and 72h） by high performance liquid chromatography（ HPLC）. Results In group A,the Ct/ C0 of lidocaine after the dog death of 72 h was 4. 74,14. 87,7. 67 in the cardiac blood,peripheral blood and brain respectively（ P〈0. 05）,the concentration of MEGX had no statistical difference（ P〉0. 05）. In group B,the Ct/ C0 of lidocaine after the dog death of72 h was 0. 36 in the cardiac blood（ P〈0. 05）. The Ct/ C0 of lidocaine was 3. 48 in the brain（ P〈0. 05）.MEGX could be detected in the liver,which had not statistical difference compared with the time since death（ P〉0. 05）. Conclusion There was a postmortem distribution of lidocaine in death dogs after subarachnoid anesthesia,there was not about MEGX.