41株致泻大肠埃希菌的药敏试验检测及结果分析

Detection and result analysis of 41 strains to xie e. coli bacteria's drug susceptibility test

目的对来源于食源性疾病病人腹泻便标本中分离的致泻大肠埃希菌进行耐药情况检测,掌握通化市食源性疾病致病菌的耐药趋势和耐药谱。方法根据CLSI推荐的微量肉汤稀释法定量测定致泻大肠埃希菌的最低抑菌浓度(minimal inhibitory concentration,MIC)。结果来源于通化市食源性疾病患者的41株致泻大肠埃希菌对环丙沙星(CIP)、庆大霉素(GEN)、氯霉素(CHL)、头孢西丁(CFX)、萘啶酸(NAL)、甲氧苄啶/磺胺甲恶唑(TMP/SMZ)、四环素(TET)、头孢噻肟(CTX)等均有不同程度的耐药。结论吉林省通化市食源性疾病患者粪便标本中分离到的致泻大肠埃希菌存在多种抗生素耐药,应高度重视和加强食源性疾病中致泻大肠埃希菌的耐药性监测,以保证食品安全和人类健康。

Objective To detect of the resistance situation of diarrhea cause diarrhea of e. coli which is isolated from foodborne disease patient specimens and to grasp the Tonghua foodborne disease trend of drug resistance of pathogenic bacteria and drug resistant spectrum. Methods According to the method of quantitative determination of trace broth dilution recommended by CLSI,cause diarrhea e. coli bacterium’s minimum bacteriostatic concentration（ minimal inhibitory concentration,MIC） were detected. Results From Tonghua foodborne disease in patients with 41 strains to xie e. h. bacterium to ciprofloxacin（ CIP） and gentamycin（ GEN） and chloramphenicol（ chlorophyll）,cefoxitin（ NAL） provided（ CFX）,nalidixic acid,methyl benzyl oxygen organism / sulfamethoxazole（ SMZ / TMP）,tetracycline（ TET）,cefotaxime（ CTX） all had different degrees of resistance. Conclusion In Tonghua foodborne disease patient stool specimens the separation to the cause of diarrhea in e. coli exist multiple antibiotic resistance,should attach great importance to and strengthen foodborne disease cause diarrhea in e. coli resistance monitoring,to ensure food safety and human health.