紫草素抑制STAT_3信号通路对人绒毛膜癌JEG-3细胞迁移和侵袭能力的影响

Effect of Shikonin on Suppressing Migrationg and Invationg of JEG-3 Cells through Inhibiting STAT_3 Signal Pathway

目的:观察紫草素抑制信号传导和转录激活因子-3(STAT_3)信号通路对人绒毛膜癌JEG-3细胞迁移和侵袭能力的影响,探讨其抗绒毛膜癌的可能机制。方法:体外培养JEG-3细胞,加入0.2、0.4μg·mL^(-1)紫草素作为实验组,阴性对照组加入等体积0.1%二甲基亚砜,甲氨蝶呤组(MTX组)作为阳性对照组加入2μg·mL^(-1)MTX。采用划痕修复实验、Transwell小室体外侵袭实验,观察紫草素对JEG-3细胞迁移和侵袭能力的影响。采用免疫组织化学分析STAT_3和酪氨酸磷酸化STAT_3(p-STAT_3)阳性细胞的表达。进一步采用Real-Time PCR分析STAT_3mRNA和pSTAT_3mRNA的表达。结果:紫草素可显著抑制JEG-3细胞迁移和侵袭能力(P<0.05或P<0.01),降低STAT_3和pSTAT_3阳性细胞的表达以及STAT_3mRNA和p-STAT_3mRNA的表达(P<0.05或P<0.01)。结论:紫草素能明显抑制JEG-3细胞迁移和侵袭能力,其机制可能与抑制STAT_3信号通路有关。

Objective：To investigate the effect of shikonin on suppressing migration and invation of JEG-3 cells through inhibiting signal transducer and activator of transcription-3（STAT3）signal pathway and discuss the possible mechanisms of anti-choriocarcinoma.Methods：JEG-3 cells were cultured in vitro and treated with shikonin at 0.2 μg·mL-1 and 0.4 μg·mL-1 concentrations as experimental group,the negative control group was treated with equal volume of 0.1% DMSO,MTX group（methotrexate group）was treated with 2 μg·mL-1 MTX as positive control group.Wound healing assay and transwell chamber invasion assay were used to detect the effect of shikonin on migration and invasion of JEG-3 cells.Immunohistochemistry was used to detect the expression of STAT3 and tyrosine phosphorylation STAT3（p-STAT3）positive cells.Real-Time PCR analysis was used to detect the expression of STAT3 mRNA and p-STAT3 mRNA.Results：Shikonin could inhibit migration and invasion of JEG-3 cells（P〈0.05 or P〈0.01）,the expression of STAT3 and p-STAT3 positive cells and the expression of STAT3 mRNA and p-STAT3 mRNA were inhibited significantly（P〈0.05 or P〈0.01）.Conclusion：Shikonin could inhibit migration and invasion of JEG-3 cells,the mechanism may be inhibition of STAT3 signal pathway