丁座草药材指纹图谱的建立及质量评价

Establisment of Control Fingerprint and Quality Assessment of Boschniakia himalaica Hook.f.et Thorns

目的:研究并建立丁座草药材的指纹图谱,为有效控制其质量提供可靠方法。方法:采用反相超高效液相色谱法,Purospher C_(18)色谱柱,乙腈-0.05%磷酸溶液梯度洗脱,流速:0.8 mL·min^(-1),检测波长:278 nm,并且采用"中药指纹图谱计算机辅助相似度评价软件"进行了指纹图谱相似度评价。结果:不同产地丁座草指纹图谱相似度较好,在指纹图谱中确定了10个共有峰,各色谱峰的分离较好,符合指纹图谱检测要求。结论:该指纹图谱检测方法重现性好,可用于丁座草药材的质量评价。

Objective： To establish the analysis method of fingerprint for Boschniakia himalaica Hook. f. et Thorns by UPLC and provide a reliable method for quality control of B. himalaica. Methods： The fingerprint of B. himalaicawas built by using Purospher C^s as column and acetoniriie-0. 05% phosphoric acid aqueous in gradient as mobile phase. The flow rate was 0. 8 mL· min- 1, and the detecting wavelength was set at 278 nm. The fingerprints of B. himalaica from different habitats were compared by Similarity Evaluation System for Chromatographic Fingerprint of CMM. Results： Samples fromdifferent habitats were of high similarities and 10 common peaks existed in thefingerprint. Each peak in the fingerprint was separated very well, satisfied to the requests of fingerprint. Conclusion ： This RP-UPLC method is repeatable in analysis of B. himalaicaand can be used in quality assessment of B. himalaica.