基于FXR探讨茵栀黄注射液利胆退黄的机制研究

Mechanism of Yinzhihuang Injection for Intrahepatic Cholestasis Based on FXR

目的:通过研究茵栀黄注射液对肝内胆汁淤积湿热证大鼠FXR、BSEP、NTCP蛋白和基因表达的影响,探讨茵栀黄注射液的作用靶点及机制。方法:51只雄性SD大鼠随机分为正常对照组(7只)、模型组(7只)、茵栀黄注射液组(7只)、GW4064组(7只)、Guggulsterone组(7只)、茵栀黄+GW4064组(8只)、茵栀黄+Guggulsterone组(8只)共7组。造模干预后观察各组大鼠肝组织FXR、BSEP、NTCP蛋白和基因的表达。结果:茵栀黄注射液可以上调FXR蛋白和基因的表达,P<0.05。FXR和BSEP在蛋白和基因表达量上存在显著相关,P<0.05。结论:茵栀黄注射液通过干预FXR上调BSEP利胆退黄治疗肝内胆汁淤积。

Objective： To study the effect of Yinzhihuang Injection on FXR,BSEP and NTCP expressions to understand the specific targets in the treatment of intrahepatic cholestasis. Methods： A total of 51 male SD rats were randomly divided into normal group（ 7）,model group（ 7）,Yinzhihuang group（ 7）,GW4064 group（ 7）,Guggulsterone group（ 7）,Yinzhihuang ＋ GW4064 group（ 8）,Yinzhihuang ＋ Guggulsterone group（ 8）. After establishing the disease and syndrome combined model after drug intervention,we observed the effect of Yinzhihuang Injection on liver＇s FXR,BSEP and NTCP expressions. Results： Yinzhihuang Injection can increase FXR protein and gene expression,P 0. 05. FXR and BSEP have a significant correlation on the protein and gene expression. Conclusion： Yinzhihuang Injection can increase the expression of FXR to raise BSEP in the treatment of intrahepatic cholestasis.