基于PB-CCD设计的丹参提取工艺的研究及主要成分含量测定

Extraction optimization of Salvia miltiorrhiza based on PB-CCD design and determination of multi-components

目的基于Plackett-Burman-central composite design(PB-CCD)设计优化丹参水提工艺。方法基于试验优化设计,采用PB-CCD设计对影响提取工艺的7种因素进行优化,采用液质联用色谱法(HPLC-ESI/MS)测定丹参水提液中丹酚酸B、丹参素钠、丹参酮ⅡA、隐丹参酮的含量作为评价指标。结果确定了基于PB-CCD设计的丹参提取的最佳工艺条件为浸泡时间0.5 h,提取时间1 h,提取温度100℃,液固比6:1,p H值7.0,提取次数3次,浓缩温度100℃。丹参药材中丹酚酸B、丹参素钠、丹参酮ⅡA、隐丹参酮的含量分别为18.91、2.142、0.1935、0.3188 mg·g^(-1)。结论基于PB-CCD设计确定的丹参提取的最佳工艺,模型预测性良好,试验设计有较高的可靠性。

Objective To optimize the extraction of effective constituents from Salvia miltiorrhiza based on PlackettBurman-central composite design（PB-CCD）. Methods Seven factors releted to the extraction were selected for optimization based on PB-CCD design. The evaluation index was contents of cryptotanshinone, tanshinone Ⅱ A, salvianolic acid and salvianic acid A sodium which were analyzed by HPLC-ESI/MS method. Results The optimum condition of extraction was soak for 0.5 h, decoction for 1 h, 6 fold solvent, pH 7.0, extraction for 3 times at 100 ℃, and concentrate at 100 ℃. The contents of salvianolic acid B, salvianic acid A sodium, tanshinone Ⅱ A and cryptotanshinone from Salvia miltiorrhiza were 18.91, 2.142, 0.1935 and 0.3188 mg·g-（-1）respectively. Conclusion The optimum model is highly predictive and reliable.