米非司酮对宫颈癌裸鼠移植瘤放疗敏感性的影响

Effects of mifepristone on the sensitivity of radiotherapy in nude mice transplanted with Hela cells

目的：探讨米非司酮对宫颈癌裸鼠移植瘤放疗敏感性的影响。方法建立人宫颈癌裸鼠移植瘤模型，将32只荷瘤裸鼠随机分为4组，分别为对照组、米非司酮组、放疗组、米非司酮＋放疗组，每组8只。定期测量肿瘤体积，绘制肿瘤生长曲线。处死裸鼠，取瘤体组织，称取各组种植瘤质量，计算抑瘤率；TUENL法检测肿瘤组织细胞凋亡。结果与对照组相比，米非司酮组、放疗组、米非司酮＋放疗组的裸鼠肿瘤质量和体积明显降低（P＜0．05），细胞凋亡指数显著提高（P＜0．05）；其中米非司酮＋放疗组瘤重及体积下降最明显（P＜0．05），细胞凋亡指数最高（P＜0．05），抑瘤率达67．74％。结论米非司酮对宫颈癌HeLa细胞裸鼠移植瘤放疗具有增敏作用。

Objective To explore the effects of mifepristone on the sensitivity of radiotherapy in nude mice trans-planted with human cervical cancer Hela cells .Methods A xenograf model was established through inoculation of Hela cells into nude mice.The mice were randomly divided into four groups （n=8）：a control group, a mifepristone group, a radiotherapy group , and a mifepristone ＋radiotherapy group .The size of xenografts was detected periodically , and a xenograft growth curve was depicted .Then, the mice were sacrificed to collect tumor tissues .The samples were weighted to calculate the inhibition rate for tumor growth .The apoptosis of tumor cells were determined by TUNEL assay .Results Compared with the control group , tumor weight and volume were significantly reduced but the apoptosis rate was re-markably increased in other three groups （P〈0.05）.The mifepristone ＋radiotherapy group showed the highest rate of apoptosis and most obvious decreases in tumor weight and volume than the mifepristone group and the radiotherapy group （P〈0.05）, with a tumor inhibitory rate of 67.74%.Conclusions Mifepristone can enhance the sensitivity of radio-therapy in nude mice transplanted with Hela cells .